IMAGING AND NANODISSECTION OF INDIVIDUAL SUPERCOILED PLASMIDS BY ATOMIC FORCE MICROSCOPY
IMAGING AND NANODISSECTION OF INDIVIDUAL SUPERCOILED PLASMIDS BY ATOMIC FORCE MICROSCOPY
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DOI：
10.1093/nar/20.3.445
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发表时间：
1992-02-11
影响因子：
14.9
通讯作者：
HENDERSON, E
中科院分区：
文献类型：
--
作者：
HENDERSON, E
原子力显微镜（AFM）用于成像沉积在云母表面的超螺旋质粒DNA在水合或干燥状态。水合质粒被扫描尖端精确地切割在仪器操作员确定的位置。小块DNA（长度100- 150nm）被切除并沉积在解剖质粒附近，表明可以通过原子力显微镜从确定的染色体位置去除和操作基因组DNA片段，这些片段在光镜下无法分辨。
The atomic force microscope (AFM) was used to image supercoiled plasmid DNA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100-150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.