Functional characterization of a Glycine soja Ca2+ATPase in salt-alkaline stress responses
Functional characterization of a Glycine soja Ca2+ATPase in salt-alkaline stress responses
复制标题
大豆 CaATP 酶在盐碱胁迫反应中的功能表征
DOI：
10.1007/s11103-015-0426-7
复制
发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Zhu, Yanming
中科院分区：
文献类型：
--
作者：
Sun, Mingzhe;Jia, Bowei;Zhu, Yanming
人们普遍认为，Ca(2+)ATP酶家族蛋白在植物环境胁迫响应中发挥重要作用。然而，到目前为止，大多数研究局限于模式植物拟南芥和水稻。非模式植物中Ca(2+)ATP酶的功能鲜有报道，尤其是其在碳酸盐碱性胁迫响应中的调控作用。因此，在本研究中，我们鉴定了大豆基因组中的P - II型Ca(2+)ATP酶家族基因，确定了它们的染色体位置和基因结构，并分析了它们的氨基酸序列和进化关系。基于上述结果，我们指出大豆Ca(2+)ATP酶存在基因重复。然后，我们研究了碳酸盐碱性胁迫下野生大豆（Glycine soja）中ACA亚家族基因的表达谱，并通过转基因苜蓿对一个代表性基因GsACA1进行了功能鉴定。我们的结果表明，苜蓿中GsACA1的过表达显著提高了植物对碳酸盐碱性和中性盐胁迫的耐受性，表现为胁迫条件下膜透性和丙二醛（MDA）含量较低，但超氧化物歧化酶（SOD）活性、脯氨酸浓度和叶绿素含量较高。综上所述，我们首次报道了来自野生大豆的P - II型Ca(2+)ATP酶GsACA1，它可以正向调控植物对碳酸盐碱性和中性盐胁迫的耐受性。
It is widely accepted that Ca(2+)ATPase family proteins play important roles in plant environmental stress responses. However, up to now, most researches are limited in the reference plants Arabidopsis and rice. The function of Ca(2+)ATPases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. Hence, in this study, we identified the P-type II Ca(2+)ATPase family genes in soybean genome, determined their chromosomal location and gene architecture, and analyzed their amino acid sequence and evolutionary relationship. Based on above results, we pointed out the existence of gene duplication for soybean Ca(2+)ATPases. Then, we investigated the expression profiles of the ACA subfamily genes in wild soybean (Glycine soja) under carbonate alkaline stress, and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses, as evidenced by lower levels of membrane permeability and MDA content, but higher levels of SOD activity, proline concentration and chlorophyll content under stress conditions. Taken together, for the first time, we reported a P-type II Ca(2+)ATPase from wild soybean, GsACA1, which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.