Amylose Stereoselectively Includes Poly(D-alanine) to Form Inclusion Complex in Vine-Twining Polymerization: A Novel Saccharide-Peptide Supramolecular Conjugate
Amylose Stereoselectively Includes Poly(D-alanine) to Form Inclusion Complex in Vine-Twining Polymerization: A Novel Saccharide-Peptide Supramolecular Conjugate
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DOI：
10.1002/macp.201500498
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发表时间：
2016-05-01
影响因子：
2.5
通讯作者：
Kadokawa, Jun-ichi
中科院分区：
文献类型：
--
作者：
Gotanda, Ryuya;Yamamoto, Kazuya;Kadokawa, Jun-ichi
在本研究中，发现直链淀粉立体选择性地包括聚（D-丙氨酸）的聚丙氨酸立体异构体在藤蔓缠绕聚合，形成相应的包合物，一种新的糖肽超分子共轭物。藤蔓缠绕聚合通过热稳定磷酸化酶催化的酶促聚合进行，其在分散于水性缓冲溶剂中的聚（D-丙氨酸）、聚（L-丙氨酸）或聚（DL-丙氨酸）的存在下产生直链淀粉。产物的X-射线衍射和H-1 NMR测定表明，包合物是由聚D-丙氨酸形成的，而使用其他立体异构体的体系不诱导包合。分析结果和客体交换实验表明，直链淀粉与聚丙氨酸的平均分子比为1：1，主体空腔部分被客体占据。
In this study, it is found that amylose stereoselectively includes poly(D-alanine) in the polyalanine stereoisomers in vine-twining polymerization to form the corresponding inclusion complex, a novel saccharide-peptide supramolecular conjugate. The vine-twining polymerization is performed by thermostable phosphorylase-catalyzed enzymatic polymerization, which produces amylose, in the presence of poly(D-alanine), poly(L-alanine), or poly(DL-alanine) dispersed in aqueous buffer solvent. The X-ray diffraction and H-1 NMR measurements of the products indicate that the inclusion complex is formed from poly(D-alanine), whereas the systems using the other stereoisomers do not induce the inclusion complexation. The analytical results and the guest exchange experiment suggest the that average molecular ratio of amylose to poly(D-alanine) is 1:1, and the host cavity is partially occupied by the guest.