Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice
Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice
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DOI：
10.1007/978-1-62703-496-8_10
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Metz, Martin
中科院分区：
文献类型：
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作者：
Doyle, Elizabeth;Trosien, Julia;Metz, Martin
全身性过敏反应的小鼠模型是研究肥大细胞功能、阐明过敏反应的病理机制以及识别和表征过敏反应的潜在治疗的重要工具。在这里，我们描述了两种全身过敏反应的小鼠模型，这是这些领域研究的关键部分。在被动模型中，在抗原攻击前24小时用抗原特异性IgE抗体致敏小鼠。在活性模型中，小鼠在攻击前18-21天用抗原致敏。在这些模型中，体温过低是过敏反应的主要可量化指标。
Mouse models of systemic anaphylaxis are important tools for the study of mast cell function, for the elucidation of the pathomechanisms of anaphylaxis, and for identifying and characterizing potential therapies for anaphylaxis. Here, we describe two murine models of systemic anaphylaxis that have been a key part of research in these areas. In a passive model, mice are sensitized with antigen-specific IgE antibody 24 h prior to antigen challenge. In an active model, mice are instead sensitized with antigen 18-21 days prior to challenge. Hypothermia serves as the primary quantifiable indicator of anaphylaxis in these models.