Requirement of histidine kinases HP0165 and HP1364 for acid resistance in Helicobacter pylori
Requirement of histidine kinases HP0165 and HP1364 for acid resistance in Helicobacter pylori
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DOI：
10.1128/iai.74.5.3052-3059.2006
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Cover, TL
中科院分区：
文献类型：
--
作者：
Loh, JT;Cover, TL
在这项研究中，我们研究了双组分信号转导系统对幽门螺杆菌耐酸性的潜在需求。与野生型菌株相比，HP0165或HP1364组氨酸激酶零突变的等基因菌株在pH 5.0下的生长能力受损。补充突变株的生长与野生型菌株相似。DNA阵列分析和转录报告基因分析表明，与亲本野生型菌株相比，HP0165和HP1364零突变株的酸反应基因转录发生了改变。这些结果表明，完整的HP0165和HP1364组氨酸激酶是幽门螺杆菌耐酸所必需的。
In this study, we investigated a potential requirement of two-component signal transduction systems for acid resistance in Helicobacter pylori. In comparison to a wild-type strain, isogenic strains with null mutations in either HP0165 or HP1364 histidine kinases were impaired in their ability to grow at pH 5.0. The growth of complemented mutant strains was similar to that of the wild-type strain. H. pylori DNA array analyses and transcriptional reporter assays indicated that acid-responsive gene transcription was altered in the HP0165 and HP1364 null mutant strains compared to the parental wild-type strain. These results indicate that intact HP0165 and HP1364 histidine kinases are required for acid resistance in H. pylori.