Isolation and genetic characterization of bovine parainfluenza virus type 3 from cattle in China
Isolation and genetic characterization of bovine parainfluenza virus type 3 from cattle in China
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DOI：
10.1016/j.vetmic.2010.11.011
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发表时间：
2011-05-05
影响因子：
3.3
通讯作者：
Xue, Fei
中科院分区：
文献类型：
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作者：
Zhu, Yuan-Mao;Shi, Hong-Fei;Xue, Fei
牛副流感病毒3型(BPIV3)是已知的幼年和成年牛呼吸道病原体中最重要的病原之一。然而，在本研究之前，尚未在中国体内检测到或分离到BPIV3。2008年，从中国地区的黄牛中分离到4株BPIV3毒株，通过RT-PCR法、核苷酸序列分析、透射电子显微镜观察、血吸附和血凝试验对其进行了鉴定。对4株BPIV3分离株的部分血凝素-神经氨酸酶(HN)基因和SD0835分离株的全基因组进行了核苷酸系统进化分析，表明这4株BPIV3株与已报道的A型(BPIV3a)和B型(BPIV3b)不同，可能是一个潜在的新基因型，暂定为C型(BPIV3c)。本研究首次报道了中国地区牛BPIV3的分离及其基因特征。(C)2010爱思唯尔B.V.保留所有权利。
Bovine parainfluenza virus type 3 (BPIV3) is one of the most important of the known viral respiratory pathogens of both young and adult cattle. However BPIV3 has not been detected or isolated in China prior to this study. In 2008, four BPIV3 strains were isolated with MDBK cells from cattle in China and characterized by RT-PCR, nucleotide sequence analysis, transmission electron microscope observation, hemadsorption and hemagglutination tests. Nucleotide phylogenetic analysis of partial hemagglutinin-neuraminidase (HN) gene for four isolates and the complete genome for the SD0835 isolate implicated that the four Chinese BPIV3 strains were distinct from the previously reported genotype A (BPIV3a) and genotype B (BPIV3b) and might be a potentially new genotype, which was tentatively classified as genotype C (BPIV3c). This is the first study to report the isolation and genetic characterization of BPIV3 from cattle in China. (C) 2010 Elsevier B.V. All rights reserved.