MOLECULAR GENETIC IDENTIFICATION OF SYMBIOTIC DINOFLAGELLATES (ZOOXANTHELLAE)
MOLECULAR GENETIC IDENTIFICATION OF SYMBIOTIC DINOFLAGELLATES (ZOOXANTHELLAE)
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DOI：
10.3354/meps071065
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发表时间：
1991-03-01
影响因子：
2.5
通讯作者：
POWERS, DA
中科院分区：
文献类型：
--
作者：
ROWAN, R;POWERS, DA
虫黄藻是一种光养甲藻，在许多海洋无脊椎动物中以内共生体的形式出现。由于传统的生物学方法没有提供有效的方法来鉴定和分类，所以人们对其共生体的自然历史知之甚少。我们将分子遗传学方法应用于人畜共患病的分类研究。利用聚合酶链式反应从少量共生(非培养)藻类中通过基因扩增可以快速获得小核糖体亚单位RNA(SsRNA)基因。然后，通过用限制性内切酶消化扩增的DNA来鉴定不同的共生线虫样虫黄藻。16种珊瑚寄主的样本产生了6个不同的共生线虫单链RNA基因：不同的夏威夷珊瑚含有不同的藻类，但同一珊瑚物种的不同个体含有难以区分的藻类。珊瑚Pocillopora Damicornis和P.meandrina似乎含有两个虫黄单链RNA基因的混合物；其他宿主表现出单一的Zocoxanthella基因型。相同的藻类基因型出现在分类上不同的宿主中。
Zooxanthellae are phototrophic dinoflagellates that occur as endosymbionts in many marine invertebrates. Because traditional biological methods have not provided useful schemes for zooxanthella identification and classification, the natural history of zooxanthella symbioses is poorly known. We have applied molecular genetic methods to zooxanthella taxonomy. Small ribosomal subunit RNA (ssRNA) genes can be rapidly obtained from small amounts of symbiotic (non-cultured) algae by gene amplification using the polymerase chain reaction. Different Symbiodinium-like zooxanthellae are then identified by digesting the amplified DNA with restriction enzymes. A sample of 16 cnidarian host species yielded 6 distinct Symbiodinium ssRNA genes: different Hawaiian corals harbored different algae, but different individuals of the same coral species harbored indistinguishable algae. The corals Pocillopora damicornis and P. meandrina appear to contain a mixture of 2 zooxanthella ssRNA genes; other hosts exhibited a single zooxanthella genotype. The same algal genotype occurs in taxonomically dissimilar hosts.