A reference bacterial genome dataset generated on the MinION™ portable single-molecule nanopore sequencer.
A reference bacterial genome dataset generated on the MinION™ portable single-molecule nanopore sequencer.
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DOI：
10.1186/2047-217x-3-22
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发表时间：
2014
期刊：
影响因子：
9.2
通讯作者：
Loman NJ
中科院分区：
文献类型：
--
作者：
Quick J;Quinlan AR;Loman NJ
MinION™是由Oxford Nanopore Technologies开发的新型便携式单分子测序仪。它的长度为4英寸，由笔记本电脑的USB 3.0端口供电。MinION™测量由DNA链与带电蛋白质纳米孔相互作用引起的电流变化。然后，这些测量可以用于推断潜在的核苷酸序列。我们提出了一个读取数据集从全基因组鸟枪测序的模式生物大肠杆菌K-12 substr。在早期访问MinION™ Access程序（MAP）期间，在MinION™器械上生成MG 1655。提供了MinION™的测序运行，一个使用R7化学（2014年7月发布）生成，一个使用R7.3（2014年9月发布）生成。提供碱基识别序列数据以证明MinION™平台产生的数据的性质，并鼓励开发用于比对、共有和变异识别、从头组装和支架化的定制方法。提供了包含HDF 5容器格式内的事件数据的FAST 5文件，以帮助开发改进的碱基调用方法。
The MinION™ is a new, portable single-molecule sequencer developed by Oxford Nanopore Technologies. It measures four inches in length and is powered from the USB 3.0 port of a laptop computer. The MinION™ measures the change in current resulting from DNA strands interacting with a charged protein nanopore. These measurements can then be used to deduce the underlying nucleotide sequence. We present a read dataset from whole-genome shotgun sequencing of the model organism Escherichia coli K-12 substr. MG1655 generated on a MinION™ device during the early-access MinION™ Access Program (MAP). Sequencing runs of the MinION™ are presented, one generated using R7 chemistry (released in July 2014) and one using R7.3 (released in September 2014). Base-called sequence data are provided to demonstrate the nature of data produced by the MinION™ platform and to encourage the development of customised methods for alignment, consensus and variant calling, de novo assembly and scaffolding. FAST5 files containing event data within the HDF5 container format are provided to assist with the development of improved base-calling methods.