Knockdown of Tripartite-59 (TRIM59) Inhibits Cellular Proliferation and Migration in Human Cervical Cancer Cells.
Knockdown of Tripartite-59 (TRIM59) Inhibits Cellular Proliferation and Migration in Human Cervical Cancer Cells.
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DOI：
10.3727/096504016x14741511303522
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发表时间：
2017-03-13
影响因子：
3.1
通讯作者：
Kuerban G
中科院分区：
文献类型：
--
作者：
Aierken G;Seyiti A;Alifu M;Kuerban G
TRIM蛋白家族是一类高度保守的蛋白质，参与多种生物学过程。TRIM 59是TRIM家族的成员之一，现已被公认为人类疾病发展和进展的关键调节因子。然而，其在人类肿瘤发生中的作用在很大程度上仍然未知。在这项研究中，TRIM 59表达对细胞增殖和迁移的影响进行了研究，在人宫颈癌细胞。采用RT-PCR和Western blot初步检测TRIM 59在临床宫颈癌组织和宫颈癌细胞中的表达。然后使用针对TRIM 59的特异性shRNA敲低宫颈癌细胞系HeLa和SiHa中TRIM 59的表达。通过MTT法和集落形成实验检测TRIM 59基因敲低对细胞增殖的影响。采用Transwell法检测TRIM 59基因敲减前后细胞的迁移和侵袭能力。我们的研究结果表明，TRIM 59的表达在宫颈癌中显著升高。TRIM 59的敲低显著抑制宫颈癌HeLa和SiHa细胞的细胞增殖和集落形成以及细胞迁移和侵袭能力。细胞周期进展分析显示TRIM 59-耗尽的细胞优选在S期积累。这些数据表明，TRIM 59是促进宫颈癌进展的潜在靶点。
The tripartite motif (TRIM) family of proteins is a class of highly conservative proteins that have been implicated in multiple processes. TRIM59, one member of the TRIM family, has now received recognition as a key regulator in the development and progression of human diseases. However, its role in human tumorigenesis has remained largely unknown. In this study, the effects of TRIM59 expression on cell proliferation and migration were investigated in human cervical cancer cells. The expression of TRIM59 in clinical cervical cancer tissues and cervical cancer cells was initially determined by RT-PCR and Western blot. Specific shRNA against TRIM59 was then employed to knock down the expression of TRIM59 in cervical cancer lines HeLa and SiHa. The effects of TRIM59 knockdown on cell proliferation was assessed by MTT assay and colony formation assay. Transwell assay was conducted to reveal cell migration and invasion abilities before and after TRIM59 knockdown. Our results showed that the expression of TRIM59 was significantly elevated in cervical cancers. Knockdown of TRIM59 significantly inhibited cell proliferation and colony formation as well as cell migration and invasion abilities in cervical cancer HeLa and SiHa cells. Cell cycle progression analysis showed that TRIM59-depleted cells preferred to accumulate in the S phase. These data suggest that TRIM59 is a potential target that promotes the progression of cervical cancer.