Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae
Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae
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DOI：
10.1007/s00253-011-3585-8
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发表时间：
2012-03-01
影响因子：
5
通讯作者：
Bashan, Yoav
中科院分区：
文献类型：
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作者：
Covarrubias, Sergio A.;de-Bashan, Luz E.;Bashan, Yoav
当淡水微藻小球藻sorokiniana和植物生长促进细菌Azoacellum brasilense部署为自由悬浮液在未灭菌的，城市污水三级污水处理，其人口显着低于他们的人口相比，在无菌废水。与此同时，天然微型动物和废水细菌的数量增加。固定化C. sorokiniana和A.小的（直径2-4 mm）聚合物海藻酸钙珠粒中的brasilense，当这些珠粒悬浮在正常废水中时，显著地增强了它们的种群。通过定量荧光原位杂交结合扫描电子显微镜和直接测量来评价珠粒内和表面上的所有微生物种群。将固定化微球浸没在废水中产生以下事件序列：（a）由废水细菌和A.在珠粒表面上产生brasilense，（B）珠粒抑制外部生物体渗透到珠粒中，（c）珠粒抑制固定化微生物释放到废水中，和（d）允许废水中铵和磷的不间断还原。这项研究表明，废水微生物种群负责减少用于废水处理的生物制剂的种群，并且在藻酸盐珠中的固定化为这些制剂提供了保护性环境，以进行不间断的三级废水处理。
When the freshwater microalga Chlorella sorokiniana and the plant growth-promoting bacterium Azospirillum brasilense were deployed as free suspensions in unsterile, municipal wastewater for tertiary wastewater treatment, their population was significantly lower compared with their populations in sterile wastewater. At the same time, the numbers of natural microfauna and wastewater bacteria increased. Immobilization of C. sorokiniana and A. brasilense in small (2-4 mm in diameter), polymer Ca-alginate beads significantly enhanced their populations when these beads were suspended in normal wastewater. All microbial populations within and on the surface of the beads were evaluated by quantitative fluorescence in situ hybridization combined with scanning electron microscopy and direct measurements. Submerging immobilizing beads in wastewater created the following sequence of events: (a) a biofilm composed of wastewater bacteria and A. brasilense was created on the surface of the beads, (b) the bead inhibited penetration of outside organisms into the beads, (c) the bead inhibited liberation of the immobilized microorganisms into the wastewater, and (d) permitted an uninterrupted reduction of ammonium and phosphorus from the wastewater. This study demonstrated that wastewater microbial populations are responsible for decreasing populations of biological agents used for wastewater treatment and immobilization in alginate beads provided a protective environment for these agents to carry out uninterrupted tertiary wastewater treatment.