Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation
Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation
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DOI：
10.1105/tpc.105.034249
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发表时间：
2005-09-01
期刊：
影响因子：
11.6
通讯作者：
Houben, A
中科院分区：
文献类型：
--
作者：
Gernand, D;Rutten, T;Houben, A
完全的单亲染色体消除发生在几个植物的种间杂交中。我们研究了小麦(Triticum Aestivum)3珍珠谷子(Pennisetum Glucum)杂交胚胎发育过程中父本染色体选择性消除的机制。所有珍珠谷子染色体在授粉后6~23d以随机顺序消失。杂交核内亲本基因组在空间上分离，待消除的珍珠小米染色质占据周边间期位置。父本染色体发生了结构重组，亲本染色体的有丝分裂行为有所不同。我们提供了一种新的染色体消除途径的证据，该途径除了涉及有丝分裂后形成的微核外，还涉及间期期间核挤出的形成。核染色质和微核染色质结构不同，微核珍珠粟染色质的异染色化和DNA碎裂是单倍化过程中的最后一步。
Complete uniparental chromosome elimination occurs in several interspecific hybrids of plants. We studied the mechanisms underlying selective elimination of the paternal chromosomes during the development of wheat ( Triticum aestivum) 3 pearl millet ( Pennisetum glaucum) hybrid embryos. All pearl millet chromosomes were eliminated in a random sequence between 6 and 23 d after pollination. Parental genomes were spatially separated within the hybrid nucleus, and pearl millet chromatin destined for elimination occupied peripheral interphase positions. Structural reorganization of the paternal chromosomes occurred, and mitotic behavior differed between the parental chromosomes. We provide evidence for a novel chromosome elimination pathway that involves the formation of nuclear extrusions during interphase in addition to postmitotically formed micronuclei. The chromatin structure of nuclei and micronuclei is different, and heterochromatinization and DNA fragmentation of micronucleated pearl millet chromatin is the final step during haploidization.