Cis-interactions between Delta and Notch modulate neurogenic signalling in Drosophila.
Cis-interactions between Delta and Notch modulate neurogenic signalling in Drosophila.
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发表时间：
1998-11
期刊：
影响因子：
4.6
通讯作者：
T. Jacobsen;K. Brennan;A. Arias;M. Muskavitch
中科院分区：
文献类型：
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作者：
T. Jacobsen;K. Brennan;A. Arias;M. Muskavitch
我们发现，发育中的刚毛器官内的神经元中 Delta 或 Serrate 的异位表达能够在多种发育环境中非自主诱导前毛原细胞转化为托莫原细胞。 Delta 诱导这些转化的频率取决于异位 Delta 表达的水平和内源性 Notch 信号通路成分的水平。当内源 Delta 水平降低时，前毛细胞对 Delta 或 Serrate 介导的转化反应更加敏感，而当内源 Delta 剂量增加时，前毛细胞对 Delta 或 Serrate 介导的转化反应更敏感，这支持了 Delta 自主干扰细胞接收任一信号的能力的假设。我们还发现，Notch 的显性失活形式 ECN 能够自主干扰细胞生成 Delta 信号的能力。当介导 Delta 和 Notch 胞外结构域之间反式相互作用的 Notch 区域从 ECN 中去除后，Delta 发出信号的能力就会恢复。我们的研究结果表明，Delta 和 Notch 之间的细胞自主相互作用可以影响细胞生成和转导 Delta 介导信号的能力。最后，我们提供的证据表明，Fringe 蛋白可以干扰发育中的刚毛器官中 Delta 和 Serrate 介导的信号传导，这与之前关于 Fringe 对发育中翅膀中 Delta 信号传导的相反影响的报道形成鲜明对比。
We find that ectopic expression of Delta or Serrate in neurons within developing bristle organs is capable of non-autonomously inducing the transformation of the pre-trichogen cell into a tormogen cell in a wide variety of developmental contexts. The frequencies at which Delta can induce these transformations are dependent on the level of ectopic Delta expression and the levels of endogenous Notch signalling pathway components. The pre-trichogen cell becomes more responsive to Delta- or Serrate-mediated transformation when the level of endogenous Delta is reduced and less responsive when the dosage of endogenous Delta is increased, supporting the hypothesis that Delta interferes autonomously with the ability of a cell to receive either signal. We also find that a dominant-negative form of Notch, ECN, is capable of autonomously interfering with the ability of a cell to generate the Delta signal. When the region of Notch that mediates trans-interactions between Delta and the Notch extracellular domain is removed from ECN, the ability of Delta to signal is restored. Our findings imply that cell-autonomous interactions between Delta and Notch can affect the ability of a cell to generate and to transduce a Delta-mediated signal. Finally, we present evidence that the Fringe protein can interfere with Delta- and Serrate-mediated signalling within developing bristle organs, in contrast to previous reports of the converse effects of Fringe on Delta signalling in the developing wing.