Novel Vip3A Bacillus thuringiensis (Bt) maize approaches high-dose efficacy against Helicoverpa zea (Lepidoptera: Noctuidae) under field conditions: Implications for resistance management.
Novel Vip3A Bacillus thuringiensis (Bt) maize approaches high-dose efficacy against Helicoverpa zea (Lepidoptera: Noctuidae) under field conditions: Implications for resistance management.
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DOI：
10.4161/gmcr.1.5.14765
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发表时间：
2010-11-01
期刊：
影响因子：
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通讯作者：
Hutchison, William D
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文献类型：
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作者：
Burkness, Eric C;Dively, Galen;Hutchison, William D
由苏云金芽孢杆菌（Bt）生产的甜玉米（Zea mays L.）转化为表达一种新的无性杀虫蛋白Vip3A（事件MIR162，先正达种子公司），在马里兰州和明尼苏达州分别进行了4个季节和2个季节的田间试验，以评估其对玉米耳虫Helicoverpa Zea （Boddie）的有效性。每年将表达Vip3A蛋白的杂交种和同时表达Cry1Ab Bt蛋白的杂交种（事件Bt11， ATTRIBUTE(), Syngenta Seeds, Inc.）与仅表达Cry1Ab的杂交种或基因相似的非Bt杂交种进行比较。除玉米蚜外，还报道了对玉米螟（Ostrinia nubilalis, Hubner）和果夜蛾（Spodoptera frugiperda， J.E. Smith）的药效。在所有年份和地点，无杀虫剂保护的非bt杂交玉米穗平均侵染率为43%至100%，每穗侵染的玉米蚜幼虫为0.24至1.74只。相比之下，在Vip3A与Cry1Ab的金字塔杂交中，没有发现幼虫，只有最小的籽粒损伤（可能是由于其他害虫）。仅表达Cry1Ab的杂交种对玉米玉米蚜的摄食造成了中等程度的伤害，由于Cry1Ab对生长有抑制作用，存活的幼虫大多局限于1龄或2龄。这些结果表明，与Cry1Ab结合的Vip3A蛋白似乎在田间条件下提供了第一个“高剂量”，并将对持续的耐药性管理有价值。
Sweet corn, Zea mays L., transformed to express a novel vegetative insecticidal protein, Vip3A (event MIR162, Syngenta Seeds, Inc..), produced by the bacterium, Bacillus thuringiensis (Bt), was evaluated over four field seasons in Maryland and two field seasons in Minnesota for efficacy against the corn earworm, Helicoverpa zea (Boddie). Hybrids expressing the Vip3A protein and pyramided in hybrids also expressing the Cry1Ab Bt protein (event Bt11, ATTRIBUTE(), Syngenta Seeds, Inc.) were compared to hybrids expressing only Cry1Ab or to genetically similar non-Bt hybrids each year. In addition to H. zea efficacy, results for Ostrinia nubilalis (Hubner) and Spodoptera frugiperda (J.E. Smith) are presented. Over all years and locations, the non-Bt hybrids, without insecticide protection, averaged between 43 and 100% ears infested with a range of 0.24 to 1.74 H. zea larvae per ear. By comparison, in the pyramided Vip3A x Cry1Ab hybrids, no larvae were found and only minimal kernel damage (likely due to other insect pests) was recorded. Hybrids expressing only Cry1Ab incurred a moderate level of H. zea feeding damage, with surviving larvae mostly limited to the first or second instar as a result of previously documented growth inhibition from Cry1Ab. These results suggest that the Vip3A protein, pyramided with Cry1Ab, appears to provide the first "high-dose" under field conditions and will be valuable for ongoing resistance management.