A chromosomal region 7p11.2 transcript map: Its development and application to the study of EGFR amplicons in glioblastoma
A chromosomal region 7p11.2 transcript map: Its development and application to the study of EGFR amplicons in glioblastoma
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DOI：
10.1093/neuonc/4.2.86
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发表时间：
2002-04-01
期刊：
影响因子：
15.9
通讯作者：
James, CD
中科院分区：
文献类型：
--
作者：
Eley, GD;Reiter, JL;James, CD
关于人类肿瘤中扩增的染色体区域的组织的累积信息表明，扩增重复单元或扩增子可以具有简单或复杂的性质。对于前者，扩增区域通常保留其天然染色体构型并涉及单个扩增靶序列。对于复杂的扩增子，扩增的DNA通常相对于它们从其进化的正常染色体区域经历实质性重组，并且经历扩增的区域可以含有多个靶序列。以前描述胶质母细胞瘤中7p11.2表皮生长因子受体（EGFR）扩增子的工作主要依赖于使用通过连锁分析和/或放射杂交作图定位的标记，已知这两种方法在试图将相对较短（
Cumulative information available about the organization of amplified chromosomal regions in human tumors suggests that the amplification repeat units, or amplicons, can be of a simple or complex nature. For the former, amplified regions generally retain their native chromosomal configuration and involve a single amplification target sequence. For complex amplicons, amplified DNAs usually undergo substantial reorganization relative to the normal chromosomal regions from which they evolve, and the regions subject to amplification may contain multiple target sequences. Previous efforts to characterize the 7p11.2 epidermal growth factor receptor (EGFR) amplicon in glioblastoma have relied primarily on the use of markers positioned by linkage analysis and/or radiation hybrid mapping, both of which are known to have the potential for being inaccurate when attempting to order loci over relatively short (