Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis
Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis
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DOI：
10.1128/jcm.40.1.198-204.2002
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发表时间：
2002-01-01
影响因子：
9.4
通讯作者：
Ando, M
中科院分区：
文献类型：
--
作者：
Eishi, Y;Suga, M;Ando, M
结节病的病因不明。分枝杆菌属在欧洲和丙酸杆菌属被怀疑。在日本被怀疑。目前的国际合作评估了结节病和疑似细菌之间可能的病因学联系。从日本的两个研究所和意大利、德国和英国的三个研究所收集了淋巴结活检样本的福尔马林固定和石蜡包埋切片，其中108例结节病患者和65例结核病患者各一份，以及86份对照样本。痤疮丙酸杆菌、颗粒丙酸杆菌、结核分枝杆菌、鸟分枝杆菌亚种的基因组副结核杆菌和大肠杆菌（作为对照）Acre通过定量实时PCR计数。除两个结节病样本外，所有样本均发现痤疮丙酸杆菌或颗粒状芽孢杆菌。M.鸟亚种未发现副结核菌。M.在0 - 9%的结节病样品中发现结核，但在65 - 100%的结核样品中发现结核。在结节病淋巴结中，痤疮丙酸杆菌和颗粒曲霉的基因组总数远远多于M。在0 ~ 60%的结核病和对照样品中发现了结核杆菌、痤疮丙酸杆菌或颗粒曲霉，但这些样品中痤疮曲霉或颗粒曲霉的基因组总数少于结节病样品中的那些。丙酸杆菌属比分支杆菌更有可能。参与结节病的病因学，不仅在日本，而且在欧洲结节病患者中。
The cause(s) of sarcoidosis is unknown. Mycobacterium spp. are suspected in Europe and Propionibacterium spp. are suspected in Japan. The present international collaboration evaluated the possible etiological links between sarcoidosis and the suspected bacterial species. Formalin-fixed and paraffin-embedded sections of biopsy samples of lymph nodes, one from each of 108 patients with sarcoidosis and 65 patients with tuberculosis, together with 86 control samples, were collected from two institutes in Japan and three institutes in Italy, Germany, and England. Genomes of Propionibacterium acnes, Propionibacterium granulosum, Mycobacterium tuberculosis, Mycobacterium avium subsp. paratuberculosis, and Escherichia coli (as the control) Acre counted by quantitative real-time PCR. Either P. acnes or A granulosum was found in all but two of the sarcoid samples. M. avium subsp. paratuberculosis was found in no sarcoid sample. M. tuberculosis was found in 0 to 9% of the sarcoid samples but in 65 to 100% of the tuberculosis samples. In sarcoid lymph nodes, the total numbers of genomes of P. acnes or A granulosum were far more than those of M. tuberculosis, P. acnes or A granulosum was found in 0 to 60% of the tuberculosis and control samples, but the total numbers of genomes of A acnes or A granulosum in such samples were less than those in sarcoid samples. Propionibacterium spp. are more likely than Mycobacteria spp. to be involved in the etiology of sarcoidosis, not only in Japanese but also in European patients with sarcoidosis.