Cellular Expression of the Monocarboxylate Transporter (MCT) Family in the Placenta of Mice
Cellular Expression of the Monocarboxylate Transporter (MCT) Family in the Placenta of Mice
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DOI：
10.1016/j.placenta.2009.11.013
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发表时间：
2010-02-01
期刊：
影响因子：
3.8
通讯作者：
Iwanaga, T.
中科院分区：
文献类型：
--
作者：
Nagai, A.;Takebe, K.;Iwanaga, T.
乳酸作为替代能量底物发挥着重要作用，特别是在葡萄糖效用降低的情况下。质子偶联单羧酸转运蛋白（MCT）对于乳酸、酮体、和其他单羧酸盐通过质膜，并可能有助于乳酸盐通过胎盘屏障的净转运。小鼠胎盘所有MCT亚型的原位杂交研究检测到从妊娠第115天开始，MCT1、MCT4和MCT9以及GLUT 1在胎盘中的强烈mRNA表达。MCT mRNA的表达在妊娠结束时强度降低，而GLUT1 mRNA的表达始终强烈。免疫组化显示，MCT1和MCT4分别位于母体侧和胎儿侧的两层合体滋养细胞。MCT的膜定向定位得到了将MCT募集到质膜上的CD 147的共存的支持。然而，MCT 1和MCT 4沿着滋养层细胞膜的亚细胞排列与人胎盘中的亚细胞排列完全相反。尽管我们不能准确解释人和小鼠胎盘之间MCT的反向定位，它可能与人类和小鼠之间乳酸来源和胎儿利用乳酸的差异有关（C）2009 Elsevier Ltd.保留所有权利
Lactate plays an important role as an alternative energy substrate, especially in conditions with a decreased utility of glucose Proton-coupled monocarboxylate transporters (MCTs) are essential for the transport of lactate, ketone bodies, and other monocarboxylates through the plasma membrane and may contribute to the net transport of lactate through the placental barrier The present study examined the expression profile and subcellular localization of MCTs in the mouse placenta. An in situ hybridization survey of all MCT subtypes detected intense mRNA expressions of MCT1, MCT4, and MCT9 as well as GLUT1 in the placenta from gestational day 115. The expression of MCT mRNAs decreased in the intensity at the end of gestation in contrast to a consistently intense expression of GLUT1 mRNA. Immunohistochemically, MCT1 and MCT4 showed a polarized localization on the maternal side and fetal side of the two cell-layered syncytiotrophoblast, respectively. The membrane-oriented localization of MCTs was supported by the coexistence of CD147 which recruits MCT to the plasma membrane However, the subcellular arrangement of MCT1 and MCT4 along the trophoblastic cell membrane was completely opposite of that in the human placenta Although we cannot exactly explain the reversed localization of MCTs between human and murine placentas, it may be related to differences between humans and mice in the origin of lactate and its utilization by fetuses (C) 2009 Elsevier Ltd. All rights reserved