Effect of recombinant bovine granulocyte colony-stimulating factor covalently bound to polyethylene glycol injection on neutrophil number and function in periparturient dairy cows
Effect of recombinant bovine granulocyte colony-stimulating factor covalently bound to polyethylene glycol injection on neutrophil number and function in periparturient dairy cows
复制标题
DOI：
10.3168/jds.2013-7242
复制
发表时间：
2014-08-01
影响因子：
3.5
通讯作者：
Roth, James A.
中科院分区：
文献类型：
--
作者：
Kimura, Kayoko;Goff, Jesse P.;Roth, James A.
奶牛通常在产犊时经历免疫功能下降，典型的是多形核中性粒细胞（PMN）功能受损和短暂的中性粒细胞减少症。这与乳腺炎、胎盘滞留和子宫炎等疾病的发病率增加有关。为了提高围产期中性粒细胞的功能能力，我们注射牛重组牛粒细胞集落刺激因子共价结合聚乙二醇（PEG rbG-CSF）两次皮下注射，约6天前产犊和产犊后24小时内。21头第二次妊娠的奶牛入组本研究，并分为2组：PEG rbG-CSF治疗组（n = 11）和生理盐水治疗对照组（n = 10）。在PEG rbG-CSF给药后，PMN数量迅速显著增加，并在实验结束时（产犊后13天）保持升高。注射后PEG rbG-CSF可显著增加中性粒细胞对髓过氧化物酶的胞吐作用。较高的髓过氧化物酶胞吐持续至少10天后产犊。PMN超氧阴离子释放和吞噬活性组间无差异。注射PEG rbG-CSF对奶牛是安全的，未观察到显著的负面影响。PEG rbG-CSF给药的最大单一效应是循环中PMN数量的急剧增加。在感染过程的早期，准备移动到感染部位的PMN数量的增加可能会提高奶牛在围产期抵御临床疾病的能力。
Dairy cows often experience decreased immune function around the time of calving, typified by impaired polymorphonuclear neutrophil (PMN) function and a transient neutropenia. This is associated with increased disease incidence, including mastitis, retained placenta, and metritis. In an attempt to improve PMN functional capacity during the periparturient period, we injected cows with recombinant bovine granulocyte colony-stimulating factor covalently bound to polyethylene glycol (PEG rbG-CSF) twice subcutaneously, about 6 d before calving and within 24 h after calving. Twenty-one cows in their second pregnancy were enrolled in this study and divided into 2 groups: PEG rbG-CSF treated (n = 11) and saline-treated controls (n = 10). The PMN numbers quickly and dramatically increased after PEG rbG-CSF administration and remained elevated through the end of the experiment (13 d after calving). Exocytosis of myeloperoxidase by stimulated PMN, which is generally decreased in periparturient cows, was markedly increased by PEG rbG-CSF after injection. Higher myeloperoxidase exocytosis persisted for at least 10 d after calving. The PMN superoxide anion release and phagocytosis activity did not differ between groups. Injection of PEG rbG-CSF was safe for cows, with no significant negative effects observed. The greatest single effect of PEG rbG-CSF administration was a dramatic increase in circulating numbers of PMN. The increased numbers of PMN ready to move to a site of infection early in the course of an infection may improve the ability of the cow to ward off clinical disease in the periparturient period.