STRUCTURE AND CHROMOSOMAL LOCATION OF THE HUMAN GRANULIN GENE
STRUCTURE AND CHROMOSOMAL LOCATION OF THE HUMAN GRANULIN GENE
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DOI：
10.1016/0006-291x(92)92349-3
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发表时间：
1992-10-15
影响因子：
3.1
通讯作者：
BATEMAN, A
中科院分区：
文献类型：
--
作者：
BHANDARI, V;BATEMAN, A
颗粒蛋白是一类富含半胱氨酸的多肽，其中一些具有生长调节活性。我们之前已经证明，颗粒蛋白是在同一前体中编码的，该前体由串联排列的七个颗粒蛋白结构域组成。在这里，我们报告了颗粒蛋白基因蛋白质编码区的染色体定位和结构组织。利用来自人-仓鼠体细胞杂交的DNA，将颗粒蛋白基因定位在17号染色体上。颗粒蛋白基因的蛋白质编码区由12个外显子组成，覆盖约3700bp。每个串联的颗粒蛋白重复序列由两个不相等的外显子编码，这是颗粒蛋白特有的配置，允许通过交替剪接形成杂交的颗粒蛋白样蛋白。
Granulins are a family of cysteine rich polypeptides some of which have growth modulatory activity. We showed previously that the granulins are encoded within the same precursor consisting of seven granulin domains arranged in tandem. Here we report the chromosomal location and structural organization of the protein coding region of the granulin gene. The granulin gene was assigned to chromosome 17 using DNA from human-hamster somatic cell hybrids. The protein-coding region of the granulin gene was shown to comprise 12 exons covering about 3700 bp. Each tandem granulin repeat is encoded by two non-equivalent exons, a configuration unique to the granulins that would permit the formation of hybrid granulin-like proteins by alternate splicing.