Eupatilin Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Inflammation and Oxidative Stress
Eupatilin Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Inflammation and Oxidative Stress
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DOI：
10.12659/msm.917406
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发表时间：
2019-11-04
影响因子：
3.1
通讯作者：
Zhao, Hongxia
中科院分区：
文献类型：
--
作者：
Liu, Haiying;Hao, Jindou;Zhao, Hongxia
背景：真叶黄素是从蒿属植物中分离得到的一种活性黄酮类化合物，具有抗炎等多种生物活性。目的：探讨优树碱对脂多糖(LPS)诱导的大鼠急性肺损伤(ALI)的影响。苏木精-伊红染色和肺湿/干比值分析大鼠肺结构损伤。采用酶联免疫吸附试验检测相关炎症因子和肺损伤标志物。用相应试剂盒分析氧化应激因素。用Western印迹和免疫组织化学方法检测肺组织中PPAR-α的表达。结果：Eupatilin可剂量依赖性地减轻脂多糖诱导的肺组织结构损伤，降低肺湿/干比。Eupatilin可降低肺表面活性蛋白(SP)-A、SP-D、炎症因子IL-6、肿瘤坏死因子(TNF)-α和单核细胞趋化蛋白(MCP)-1水平。脂多糖可促进一氧化氮(NO)的产生，增加丙二醛(MDA)和乳酸脱氢酶(LDH)的生成，降低超氧化物歧化酶(SOD)的活性，而Eupatilin呈浓度依赖性地逆转这种作用。结论：尤帕替林通过浓度依赖的方式抑制炎症反应和氧化应激，从而减轻内毒素诱导的ALI，其机制可能与激活PPAR-α密切相关。
Background: Eupatilin, an active flavone separated from Artemisia species, has various biological activity such as anti-inflammatory activity. The aim of the present study was to find out the influence of eupatilin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats.Material/Methods: The administration of LPS was used to induce ALI; eupatilin was given 1 hour before the LPS administration. Lung structural damage of rats was analyzed by hematoxylin and eosin staining and the wet/dry lung ratio. The related inflammatory factors and lung injury markers were examined by enzyme-linked immunosorbent assay. The oxidative stress factors were analyzed by corresponding kits. The expression of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) was assayed by western blot and immunohistochemical staining.Results: The results showed that eupatilin alleviated LPS-induced structural damage and decreased the wet/dry lung ratio concentration-dependently. Eupatilin decreased the level of surfactant protein (SP)-A, SP-D, and inflammatory factors such as interleukin (IL)-6, tumor necrosis factor (TNF)-alpha, and monocyte chemo-attractant protein (MCP)-1. LPS trigged nitric oxide (NO) generation, improved the production of malondialdehyde (MDA) and lactate dehydrogenase (LDH) and decreased the activity of superoxide dismutase (SOD), which were reversed when rats treated with eupatilin in a concentration-dependent way. Besides, the expression of PPAR-alpha was increased under the treatment of eupatilin.Conclusions: Collectively, eupatilin alleviated LPS-induced ALI through inhibiting inflammation and oxidative stress in a con-centration-dependent way, which was likely to be closely related with the activation of PPAR-alpha.