A new approach to the measurement of glycerol turnover.
A new approach to the measurement of glycerol turnover.
复制标题
测量甘油周转率的新方法。
DOI：
10.1139/y78-148
复制
发表时间：
1978
影响因子：
2.1
通讯作者：
G. Steiner
中科院分区：
文献类型：
--
作者：
M. Kallai;K. Norwich;G. Steiner
这些研究描述了一种新的方法，使用单次推注标记甘油，以测量甘油周转。在一系列利用单个动物的实验中，将这种新方法与以前使用的恒定示踪剂输注方法进行了比较。两者给出了相同的结果。在推注法中，一旦确定了最大甘油比活度的时间，就可以使用在非常短的时间跨度内获得的非常少的额外数据点计算甘油转换率。这种简化是可能的，因为发现甘油比活度的衰减符合比活度对数对时间对数图上的直线。这种新的方法应简化甘油营业额的测量，并应允许甘油转化为其他最终产品的速率的测定。
These studies describe a new method, using a single bolus injection of labelled glycerol, to measure glycerol turnover. In a series of experiments utilizing single animals, this new approach was compared with the previously used method of constant tracer infusion. The two gave identical results. In the bolus method, once the time of maximum glycerol specific activity had been established, glycerol turnover could be calculated using very few additional data points obtained over a very short time span. This simplification was possible because the decay of glycerol specific activity was found to conform to a straight line on a plot of the logarithm of the specific activity versus the logarithm of the time. This new approach should simplify the measurement of glycerol turnover and should permit the determination of the rate of glycerol transformation to other end products.