STRUCTURAL STUDIES OF CELL-WALL POLYSACCHARIDES FROM BIFIDOBACTERIUM-BREVE YIT-4010 AND RELATED BIFIDOBACTERIUM SPECIES

STRUCTURAL STUDIES OF CELL-WALL POLYSACCHARIDES FROM BIFIDOBACTERIUM-BREVE YIT-4010 AND RELATED BIFIDOBACTERIUM SPECIES
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DOI:
10.1093/oxfordjournals.jbchem.a122188
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发表时间:
1987-12-01
影响因子:
2.7
通讯作者:
AZUMA, I
AZUMA, I
中科院分区:
生物学4区
文献类型:
--
作者:
HABU, Y;NAGAOKA, M;AZUMA, I

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对5种双歧杆菌和8株双歧杆菌的细胞壁进行了化学成分分析。这些细胞壁被证明是由肽聚糖和多糖部分。在同一种植物的细胞壁制备物中,观察到中性糖含量和磷含量的一些变化。对4株双歧杆菌(B.两歧型YIT 4007、B. B.短YIT 4010,B。编号YIT 4025和B。longum ATCC 15707)进行纯化并分析其化学结构。多糖从B中提取的多糖通过GLC、~ 1H-和~(13)C-NMR光谱分析、甲基化、Smith降解和乙酰解等方法对该多糖进行了详细的分析,结果表明该多糖的重复单元结构如下:
The chemical compositions of the cell walls obtained from 8 strains and in 5 species of Bifidobacterium were analyzed. These cell walls were shown to be composed of peptidoglycan and polysaccharide moieties. Some variations with respect to contents of neutral sugars and content of phosphorus were observed with some cell wall preparations from the same species. The neutral polysaccharides in cell walls of 4 strains of Bifidobacterium (B. bifidum YIT 4007, B. breve YIT 4010, B. infantis YIT 4025, and B. longum ATCC 15707) were purified and their chemical structures were analyzed. One of these polysaccharides, obtained from B. breve YIT 4010, was analyzed in detail by GLC, 1H- and 13C-NMR spectroscopic analyses, methylation, Smith degradation and acetolysis, and the results suggested the following structure for the repeating unit of the polysaccharide is presented.