Description of Eikenella halliae sp. nov. and Eikenella longinqua sp. nov., derived from human clinical materials, emendation of Eikenella exigua Stormo et al. 2019 and emendation of the genus Eikenella to include species which are strict anaerobes
Description of Eikenella halliae sp. nov. and Eikenella longinqua sp. nov., derived from human clinical materials, emendation of Eikenella exigua Stormo et al. 2019 and emendation of the genus Eikenella to include species which are strict anaerobes
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DOI：
10.1099/ijsem.0.004150
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Bernier, Anne-Marie
中科院分区：
文献类型：
--
作者：
Bernard, Kathryn A.;Burdz, Tamara;Bernier, Anne-Marie
嗜血杆菌、聚集性杆菌、心脏杆菌。Eikenella，Kingella(Hacek)属Eikenella多年来一直只有一个种，即腐蚀性Eikenella。2019年11月，在挪威从脑脓肿和血培养中康复后被描述为甜菜艾肯氏菌。巧合的是，来自17名加拿大患者的22种类似艾肯氏菌的革兰氏阴性细菌的特征一直在进行中。来自5名患者的7株分离物被确凿地鉴定为腐蚀性肠杆菌。其中一种(NML 120819)被认为代表了与腐蚀性埃希氏菌关系最密切的艾肯氏杆菌属的一种。通过16S rRNA基因测序，14株菌株与腐蚀性肠杆菌的相似性为97.6%~98.8%，基因组分析显示，14个菌株形成了3个不同的类群。最大的一组含有来自8名从血液、脑、骨和其他脓肿中恢复的患者的10株厌氧菌株；重新评估后，发现这一组与甜菜夜蛾最一致。第二个兼性厌氧Glade包括来自一名患者的两个眼部分离株和另一名患者的窦部分离株。第三分类群由单一的严格厌氧血培养分离物组成。新的分类群和E腐蚀物一样，在生物化学上反应很差，很难从表型和化学分类上相互区分，包括通过细胞脂肪酸。用MALDI-TOF(Bruker)和全基因组测序对分离株进行进一步鉴定。DNAG+C含量相似(55.38~58.53摩尔%)，大小在1.82~2.54 Mb之间。本文对Eikenella属和Eikenella exigua种进行了订正，并描述了Eikenella halliae sp.11月NML 130454(T)(=1MG 30894(T)=NCTC 14180(T))；NML02-A-017(T)(=1MG 30296(T)=NCTC 14179(T))。
The Haemophilus, Aggregatibacter, Cardiobacterium. Eikenella, Kingella (HACEK) group genus Eikenella contained a single species, Eikenella corrodens, for many years. In November 2019, Eikenella exigua was described after recovery from a brain abscess and blood culture in Norway. Coincidentally, characterization of 22 Gram-negative bacteria resembling Eikenella from 17 Canadian patients had been underway. Seven isolates from five patients were conclusively identifiable as E. corrodens. One (NML 120819) was deemed to represent a species of the genus Eikenella most closely related to E. corrodens. Fourteen isolates had 97.6 to 98.8% similarities to E. corrodens by 16S rRNA gene sequencing, forming three distinct groups by genome analyses. The largest contained ten anaerobic isolates from eight patients recovered from blood, brain, bone and other abscesses; upon re-evaluation, this group was found to be most consistent with E. exigua. A second facultatively anaerobic Glade consisted of two ocular isolates from one patient and a sinus isolate from a second patient. The third taxon consisted of a single strictly anaerobic blood culture isolate. The novel taxa, like E corrodens, were poorly reactive biochemically and difficult to discern from each other phenotypically and chemotaxonomically, including by cellular fatty acids. MALDI-TOF (Bruker) and wholegenome sequencing were used to further characterize isolates. Draft genomes for the strains had similar DNA G+C contents (55.38-58.53 mol%) while sizes varied from 1.82 Mb to 2.54 Mb. We propose here emendations of the genus Eikenella and the species Eikenella exigua, as well as describing Eikenella halliae sp. nov. NML 130454(T) (=LMG 30894(T)=NCTC 14180(T)) and Eikenella Ionginqua sp nov. NML 02-A-017(T) (=LMG 30296(T)=NCTC 14179(T)), on the basis of these findings.