Inhibition of carcinogen-altered rat tracheal epithelial cells by normal epithelial cell-conditioned medium.
Inhibition of carcinogen-altered rat tracheal epithelial cells by normal epithelial cell-conditioned medium.
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DOI：
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发表时间：
1986-02
期刊：
影响因子：
11.2
通讯作者：
M. Terzaghi-Howe;C. Mckeown
中科院分区：
文献类型：
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作者：
M. Terzaghi-Howe;C. Mckeown
越来越多的证据表明，正常细胞可以抑制致癌物改变的细胞的生长和/或影响肿瘤表型的进展。在体内完整大鼠气管组织和大鼠气管上皮细胞培养物中均观察到该效应。在培养物中致癌物改变的细胞的抑制似乎与在原代培养物中由正常气管上皮细胞或食管上皮细胞产生的酸和热稳定的、二硫苏糖醇敏感的、不可透析的蛋白质的产生有关。发现其最佳地由正常上皮细胞的3-4周龄培养物产生。在1：4稀释的正常细胞条件培养基存在下，敏感细胞系的殖民地形成效率降低5倍。该抑制剂的生物化学性质与β型转化生长因子相关的生物化学性质相似。
There is mounting evidence that normal cells can either inhibit the growth of carcinogen-altered cells and/or affect progression to a neoplastic phenotype. This effect(s) has been observed both in vivo in intact rat tracheal tissues and in rat tracheal epithelial cell cultures. The inhibition of carcinogen-altered cells in culture appears to be associated with the production of an acid and heat stable, dithiothreitol sensitive, nondialyzable protein produced by normal tracheal epithelial cells or esophageal epithelial cells in primary culture. It was found to be optimally produced by 3-4-week-old cultures of normal epithelial cells. In the presence of a 1:4 dilution of normal cell conditioned medium, the colony forming efficiency of a sensitive cell line is decreased 5-fold. Biochemical properties of the inhibitor are similar to those associated with type beta transforming growth factor.