Identifying single nucleotides by tunnelling current
Identifying single nucleotides by tunnelling current
复制标题
DOI：
10.1038/nnano.2010.42
复制
发表时间：
2010-04-01
影响因子：
38.3
通讯作者：
Kawai, Tomoji
中科院分区：
文献类型：
--
作者：
Tsutsui, Makusu;Taniguchi, Masateru;Kawai, Tomoji
医学研究的一个主要目标是开发一种能够以低成本阅读整个人类基因组的DNA测序技术（1-4）。最近，有人提出可以通过测量DNA分子中单个核苷酸的电子传输特性来进行DNA测序（5）。在这里，我们报告使用两个可配置的纳米电极的单核苷酸的电检测，并显示通过单核苷酸的电子传输发生隧道效应。我们还展示了基于电导率的核苷酸统计识别，从而为基于电子传输测量的DNA测序技术提供了实验基础。
A major goal in medical research is to develop a DNA sequencing technique that is capable of reading an entire human genome at low cost(1-4). Recently, it was proposed that DNA sequencing could be performed by measuring the electron transport properties of the individual nucleotides in a DNA molecule(5). Here, we report electrical detection of single nucleotides using two configurable nanoelectrodes and show that electron transport through single nucleotides occurs by tunnelling. We also demonstrate statistical identification of the nucleotides based on their electrical conductivity, thereby providing an experimental basis for a DNA sequencing technology based on measurements of electron transport.