Chromium (VI) uptake and tolerance potential in cotton cultivars: effect on their root physiology, ultramorphology, and oxidative metabolism.
Chromium (VI) uptake and tolerance potential in cotton cultivars: effect on their root physiology, ultramorphology, and oxidative metabolism.
复制标题
DOI：
10.1155/2014/975946
复制
发表时间：
2014
影响因子：
--
通讯作者：
Zhu SJ
中科院分区：
文献类型：
--
作者：
Daud MK;Mei L;Variath MT;Ali S;Li C;Rafiq MT;Zhu SJ
铬（Cr）是一种有毒的污染物，需要使用植物修复技术来去除。本研究以转基因抗虫棉J208、Z905及其杂交种ZD 14为材料，采用多生物标记技术研究了它们对铬的吸收和耐性。试验采用4种不同浓度的铬（CK、10、50和100 μM）。在100 μM浓度下，Cr显著降低了根/冠长度、次生根数量以及根鲜生物量和干生物量。在10、50和100 μM的铬胁迫下，杂交种ZD 14的铬积累量均高于其亲本J208和Z905。铬转运量均小于1。100 μM Cr的超微结构研究显示，细胞核和空泡数量增加，细胞死亡部分（空泡/细胞壁）中存在Cr致密颗粒。丙二醛（MDA），过氧化氢（H2 O2），总可溶性蛋白，超氧化物歧化酶（SOD），过氧化物酶（POD），抗坏血酸过氧化物酶（APX），过氧化氢酶（CAT），谷胱甘肽还原酶（GR）作为一个整体上调与铬水平升高。根系对铬的吸收、代谢加速以及细胞死亡部位对铬的固存表明这些棉花品种具有较强的耐铬性和积累能力。
Chromium (Cr) is present in our environment as a toxic pollutant, which needs to be removed using phytoremediation technology. In present study, two transgenic cotton cultivars (J208, Z905) and their hybrid line (ZD14) were used to explore their Cr uptake and tolerance potential using multiple biomarkers approach. Four different levels of Cr (CK, 10, 50, and 100 μM) were applied. Cr caused a significant reduction in root/shoot length, number of secondary roots, and root fresh and dry biomasses at 100 μM. Cr accumulated more in roots and was found higher in hybrid line (ZD14) as compared with its parent lines (J208, Z905) at all Cr stress levels (10, 50, and 100 μM). Cr translocation was less than 1 in all cultivars. Ultrastructural studies at 100 μM Cr showed an increase in number of nuclei and vacuoles and presence of Cr dense granules in dead parts of the cell (vacuoles/cell wall). Malondialdehyde (MDA), hydrogen peroxide (H2O2), total soluble proteins, superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR) as a whole were upregulated with elevated levels of Cr. Higher Cr uptake by roots, accelerated metabolism, and Cr sequestration in dead parts of the cell indicate that these cotton cultivars can be useful for Cr accumulation and tolerance.