A Case of Moderately Severe COVID-19 in a Healthcare Worker in Russia: Virus Isolation and Full Genome Sequencing.
A Case of Moderately Severe COVID-19 in a Healthcare Worker in Russia: Virus Isolation and Full Genome Sequencing.
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DOI：
10.7759/cureus.13733
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发表时间：
2021-03-06
期刊：
影响因子：
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通讯作者：
Gushchin V
中科院分区：
文献类型：
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作者：
Shchetinin AM;Tsyganova EV;Protsenko DN;Botikov AG;Gushchin V
从临床和分子流行病学的角度来看，2019 年冠状病毒病（COVID-19）大流行可能是历史上研究最多的一场流行病。尽管如此，有关严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 病毒基因型与其引起的 COVID-19 症状之间相关性的数据仍然很少。在本报告中，我们介绍了俄罗斯莫斯科一名医护人员的中度严重 COVID-19 病例，并补充了有关其病原体表型的体外和全基因组特征数据。这名 44 岁的男性医护人员经常与 COVID-19 患者进行专业接触，被诊断出病毒性肺炎而住院，很快开始出现发烧、阵发性干咳、嗅觉丧失，胸部 CT 扫描发现双肺出现典型的磨玻璃样混浊。 COVID-19 诊断通过实时定量聚合酶链反应 (qRT-PCR)、免疫层析和免疫化学发光检测进行验证。该患者接受了羟氯喹、阿奇霉素、扑热息痛和依诺肝素治疗，发病两周后康复。使用 Vero E6 细胞系从发病第五天采集的鼻咽拭子样本中成功分离出该病毒，并表现出明显的细胞病变效应（CPE），细胞培养基中的病毒滴度达到 106 TCID50/ml。获得了该病毒分离株的完整基因组序列，并鉴定出与 Wuu-Hu-1 参考基因组相比的 8 个核苷酸和 5 个氨基酸突变。病毒分离株属于 GR/20B/B.1.1 遗传谱系（分别为 GISAID、Nextstrain、Pangolin 命名法）——迄今为止在俄罗斯发现的最流行的基因型。
The coronavirus disease 2019 (COVID-19) pandemic is probably the most studied one in history from both clinical and molecular-epidemiological perspectives. Nonetheless, data on the correlation between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral genotypes and COVID-19 symptoms caused by them are still scarce. In this report, we present a moderately severe COVID-19 case in a healthcare worker in Moscow, Russia, supplemented with the data on its causative agent's phenotype regarding in vitro and full-genome characterization. The 44-year-old male healthcare worker who had frequent professional contacts with COVID-19 patients was hospitalized with a viral pneumonia diagnosis and soon started to exhibit fever, dry paroxysmal cough, loss of smell, and typical ground-glass opacities found in both lungs on chest CT scans. The COVID-19 diagnosis was verified by real-time quantitative polymerase chain reaction (qRT-PCR), immunochromatography, and immunochemiluminescent assays. The patient was treated with hydroxychloroquine, azithromycin, paracetamol, and enoxaparin, leading to his recovery after two weeks from the disease onset. The virus was successfully isolated from the nasopharyngeal swab sample taken on the fifth day of the disease onset using the Vero E6 cell line and exhibited a pronounced cytopathic effect (CPE) with a viral titer reaching 106 TCID50/ml in the cell culture medium. The full genome sequence of the viral isolate was obtained and 8 nucleotide and 5 amino acid mutations compared to the Wuhan-Hu-1 reference genome were identified. Viral isolate belonged to GR / 20B / B.1.1 genetic lineage (GISAID, Nextstrain, Pangolin nomenclatures, respectively) - the most prevalent genotype found in Russia to date.