STUDIES OF EXTRACELLULAR AND INTRACELLULAR BACTERIAL DEOXYRIBONUCLEIC ACIDS
STUDIES OF EXTRACELLULAR AND INTRACELLULAR BACTERIAL DEOXYRIBONUCLEIC ACIDS
复制标题
DOI：
10.1099/00221287-19-3-522
复制
发表时间：
1958-01-01
期刊：
影响因子：
--
通讯作者：
CUNNINGHAM, LS
中科院分区：
文献类型：
--
作者：
CATLIN, BW;CUNNINGHAM, LS
分析了从细胞外和细胞内位置获得的高度聚合的细菌脱氧核糖核酸（DNA）。从金黄色葡萄球菌（Staphylococcus aureus）和金黄色葡萄球菌（S.表皮杆菌）是具有高腺嘌呤和胸腺嘧啶含量的类型;另外两种（粪产碱杆菌和荧光假单胞菌）具有高鸟嘌呤和胞嘧啶含量。对于每种微生物，细胞外DNA的组成基本上与细胞内DNA的组成相同。研究了与3种菌株培养物中DNA胞外积累相关的条件。S.金黄色葡萄球菌精心制作的脱氧核糖核酸酶（DNase），需要钙离子和高pH值的活动。在C. pH 6和钙缺乏，DNA酶失活，DNA粘液积累。在荧光假单胞菌的培养物中，一种特异性的核糖核酸酶敏感的DNA酶抑制剂保护粘液DNA免受培养物DNA酶的解聚。在A.此外，在粪肠球菌中，可能出现DNA粘液和低浓度的DNA酶。这种酶，通过研究其对小牛胸腺DNA从干燥的纤维制备的解决方案的行动检测，被激活的各种阳离子的pH范围内的文化。然而，与其他DNA酶不同的是，它几乎没有攻击粘层DNA的能力，而粘层DNA可能更接近于天然的。
Highly polymerized bacterial deoxyribonucleic acids (DNAs) obtained from extracellular and from intracellular locations were analysed. DNAs from two strains (Staphylococcus aureus and S. epidermidis) were of the type having high adenine and thymine contents; two others (Alcaligenes faecalis and Pseudomonas fluorescens) had a high content of guanine and cytosine. For each micro-organism the composition of extracellular DNA was essentially the same as that of the intracellular DNA. Conditions associated with the extracellular accumulation of DNA in cultures of 3 strains were investigated. S. aureus elaborated a deoxyribonuclease (DNase) which required calcium ion and high pH for activity. In a culture medium of c. pH 6 and insufficient calcium, the DNase was inactive and DNA slime accumulated. In cultures of P. fluorescens a specific ribonuclease-sensitive DNase inhibitor protected slime DNA from depolymerization by culture DNase. In cultures of A. faecalis, also, both a DNA slime and a low concentration of DNase may occur. This enzyme, detected by studying its action against solutions of calf thymus DNA prepared from dried fibres, was activated by various cations within the pH range of cultures. Unlike other DNases, however, it has little capacity to attack the slime-layer DNA, which presumably is more nearly native.