Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.
Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.
复制标题
大鼠肠维生素D依赖性钙结合蛋白cDNA和染色体基因的分子克隆。
DOI：
10.1073/pnas.84.17.6108
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Darwish,HM;Krisinger,J;Strom,M;DeLuca,HF
通过对大鼠肠道cDNA文库的筛选，获得了维生素D依赖大鼠肠道钙结合蛋白的编码基因。该基因全长406个核苷酸，似乎包含了该基因的所有序列。该基因包括整个蛋白质编码区。它由237个核苷酸组成，编码79个氨基酸，包括起始的蛋氨酸，在5‘端和3’端分别有62个和107个非编码核苷酸。利用克隆的cDNA，我们从大鼠肝脏基因组文库中分离了一个基因组克隆。利用人工合成的寡核苷酸进行限制图谱和Southern分析，将该基因定位于一个4.0kb的HindIII片段。
A cDNA encoding the vitamin D-dependent rat intestinal calcium-binding protein has been isolated by screening a rat intestinal cDNA library. The cDNA is 406 nucleotides long and appears to contain all the sequences of the mRNA. The cDNA includes the entire protein coding region. It consists of 237 nucleotides coding for 79 amino acids, including the starting methionine, flanked by 62 and 107 noncoding nucleotides at the 5' and 3' ends, respectively. Using the cloned cDNA, we have isolated a genomic clone from a rat liver genomic library. Restriction mapping and Southern analysis using synthetic oligonucleotides localized the gene to a 4.0-kilobase-pair HindIII fragment.