ANTIGEN PRESENTATION AND TUMOR-CYTOTOXICITY BY INTERFERON-GAMMA-TREATED MICROGLIAL CELLS
ANTIGEN PRESENTATION AND TUMOR-CYTOTOXICITY BY INTERFERON-GAMMA-TREATED MICROGLIAL CELLS
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DOI：
10.1002/eji.1830170909
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发表时间：
1987-09-01
影响因子：
5.4
通讯作者：
FONTANA, A
中科院分区：
文献类型：
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作者：
FREI, K;SIEPL, C;FONTANA, A
在这项研究中，从新生小鼠脑细胞培养物中分离的小胶质细胞进行了表征，并研究了形态，它们对生长因子的反应及其功能特性。小胶质细胞是吞噬细胞，含有非特异性酯酶活性，并表达Fc（IgG 1/2b）和3型补体受体。扫描电子显微镜显示，在类似于脑组织中存在两种类型的小胶质细胞的文化：阿米巴样和分枝型，这两种类型都显示出相似的外观，通过透射电子显微镜。白细胞介素3和粒细胞-巨噬细胞集落刺激因子是粒细胞-巨噬细胞集落刺激因子的强效生长因子，粒细胞-巨噬细胞集落刺激因子是培养的小胶质细胞的强效生长因子。细胞对主要组织相容性抗原复合物的II类抗原（Ia）呈阴性。然而，在用干扰素-γ治疗后，（IFN-γ）当在卵清蛋白特异性Ia限制性辅助性T细胞上测试时，小胶质细胞变成Ia+，并作为抗原呈递细胞发挥功能。此外，暴露于IFN-γ的小神经胶质细胞内毒素产生肿瘤细胞毒性并产生肿瘤坏死因子α。总之，小胶质细胞具有巨噬细胞谱系细胞的特征。
In this study microglial cells isolated from brain cell cultures of newborn mice were characterized and investigated for morphology, their responses to growth factors and their functional properties. The microglial cells were phagocytic, contained non-specific esterase actvity and expressed Fc (IgG1/2b) and type-3 complement receptors. Scanning electron microscopy revealed that in analogy to brain tissue two types of microglial cells are present in the cultures: the ameboid and the ramified type which both display similar appearance by transmission electron microscopy. Interleukin 3 and the granulocyte-macrophage colony-stimulating factor were potent growth factors for the granulocyte-macrophage colony-stimulating factor were potent growth factors for the cultured microglial cells. The cells were negative for class II antigens (Ia) of the major histocompatibility antigen complex. However, upon treatment with interferon-.gamma. (IFN-.gamma.) microglial cells became Ia+ and functioned as antigen-presenting cells when tested on ovalbumin-specific Ia-restricted helper T cells. Furthermore, microglial cells exposed to IFN-.gamma. and endotoxin developed tumor cell cytotoxicity and produced tumor necrosis factor .alpha.. Taken together, microglial cells share the characteristics of cells of the macrophage lineage.