An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome
An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome
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DOI：
10.1016/j.fertnstert.2012.01.122
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发表时间：
2012-05-01
影响因子：
6.7
通讯作者：
Brennan, Lorraine
中科院分区：
文献类型：
--
作者：
Wallace, Martina;Cottell, Evelyn;Brennan, Lorraine
目的：确定卵泡液的代谢组学分析是否可以证明是一种用于选择存活卵母细胞和胚胎的有用的无创技术。设计：对从体外受精 (IVF) 患者收集的卵泡液进行基于质子核磁共振 (H-1 NMR) 的代谢组学分析。地点：一所大学研究中心和一家私人生育诊所。患者：58 名接受 IVF 治疗的女性。干预措施：收集卵泡液主要结果指标：代谢组学特征、卵母细胞发育潜力和胚胎活力的评估。结果：卵母细胞产生未能卵裂的受精卵 (n = 9) 的卵泡液的代谢组学特征与卵母细胞发育成早期卵裂阶段胚胎的情况明显不同。区分代谢物包括葡萄糖、乳酸、胆碱/磷酸胆碱和脂蛋白。对随后 β 人绒毛膜促性腺激素呈阳性的女性 (n = 10) 与妊娠失败的女性 (n = 12) 的卵泡液进行比较，发现与周期结果相关的代谢差异。结论：卵泡液代谢物成分的差异与人类卵母细胞的发育能力相关。因此，卵泡液的代谢组学分析可能被证明是配子/胚胎选择中的一项重要技术。 （Fertil Steril (R) 2012；97:1078-84。(C) 2012，美国生殖医学会。）
Objective: To determine whether metabolomic analysis of follicular fluid could prove a useful noninvasive technique for the selection of viable oocytes and embryos.Design: Metabolomic analysis based on proton nuclear magnetic resonance (H-1 NMR) performed on follicular fluid collected from in vitro fertilization (IVF) patients.Setting: A university research center and a private fertility clinic.Patient(s): Fifty-eight women undergoing IVF treatment.Intervention(s): Follicular fluid collected at the time of oocyte retrieval.Main Outcome Measure(s): Metabolomic profile, assessment of oocyte developmental potential and embryo viability.Result(s): The metabolomic profile of follicular fluid from follicles where the oocyte resulted in a fertilized egg that failed to cleave (n = 9) was distinctly different from that where oocytes developed into early cleavage-stage embryos. Discriminating metabolites included glucose, lactate, choline/phosphocholine, and lipoproteins. Comparison of follicular fluid from women who subsequently had a positive beta human chorionic gonadotropin (n = 10) to those who were unsuccessful in achieving a pregnancy (n = 12) revealed metabolic differences that were correlated to cycle outcome.Conclusion(s): Differences in the metabolite composition of follicular fluid correlate with the developmental competence of the human oocyte. Therefore, metabolomic profiling of follicular fluid may prove to be an important technique in gamete/embryo selection. (Fertil Steril (R) 2012;97:1078-84. (C) 2012 by American Society for Reproductive Medicine.)