Anovulation in Cyclooxygenase-2-Deficient Mice Is Restored by Prostaglandin E2 and Interleukin-1β.
Anovulation in Cyclooxygenase-2-Deficient Mice Is Restored by Prostaglandin E2 and Interleukin-1β.
复制标题
DOI：
10.1210/endo.140.6.6715
复制
发表时间：
1999-06
期刊：
影响因子：
4.8
通讯作者：
B. Davis;D. Lennard;Christopher A. Lee;H. Tiano;S. Morham;W. Wetsel;R. Langenbach
中科院分区：
文献类型：
--
作者：
B. Davis;D. Lennard;Christopher A. Lee;H. Tiano;S. Morham;W. Wetsel;R. Langenbach
携带前列腺素生成所必需的两种环加氧酶（COX）同工酶中任何一种的无效突变的小鼠表现出几种同种型特异性的生殖异常。缺乏COX-1的小鼠具有生育能力，但幼仔活力下降，而缺乏COX-2的小鼠无法排卵，并具有异常的着床和蜕膜反应。本研究确定了每种 COX 同工酶在下丘脑、垂体和卵巢功能中的具体贡献，并建立了 COX-2 缺陷小鼠无排卵综合征的病理学和挽救方法。在 COX-1 和 COX-2 缺陷小鼠中，垂体促性腺激素选择性增加，而下丘脑 LHRH 和血清促性腺激素水平与野生型动物相似 (+/+)。三种基因型之间的血清雌激素或孕激素没有显着差异。 PMSG 和 hCG 的外源性促性腺激素刺激使野生型和 COX 中卵巢 PGE2 水平增加了 4 倍......
Mice carrying a null mutation for either of the two cyclooxygenase (COX) isoenzymes, necessary for prostanoid production, exhibit several isotype-specific reproductive abnormalities. Mice deficient in COX-1 are fertile but have decreased pup viability, whereas mice deficient in COX-2 fail to ovulate and have abnormal implantation and decidualization responses. The present study identifies the specific contribution of each COX isoenzyme in hypothalamic, pituitary, and ovarian function and establishes the pathology and rescue of the anovulatory syndrome in the COX-2-deficient mouse. In both COX-1- and COX-2-deficient mice, pituitary gonadotropins were selectively increased, whereas hypothalamic LHRH and serum gonadotropin levels were similar to those in wild-type animals (+/+). No significant differences in serum estrogen or progesterone were noted among the three genotypes. Exogenous gonadotropin stimulation with PMSG and hCG produced a comparable 4-fold increase in ovarian PGE2 levels in wild-type and COX...