Structural alteration of cell wall pectins accompanies pea development in response to cold
Structural alteration of cell wall pectins accompanies pea development in response to cold
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DOI：
10.1016/j.phytochem.2014.04.011
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发表时间：
2014-08-01
期刊：
影响因子：
3.8
通讯作者：
Rayon, Catherine
中科院分区：
文献类型：
--
作者：
Baldwin, Laetitia;Domon, Jean-Marc;Rayon, Catherine
研究了豌豆（Pisum sativum）细胞壁代谢对低温的响应，并对抗冻敏感基因型“Terese”和抗冻基因型“Champagne”进行了比较。从冷驯化和非驯化植物的托叶分离的细胞壁表明，低温诱导含有木糖，阿拉伯糖，半乳糖和半乳糖醛酸残基的聚合物的变化。在耐受性栽培品种香槟中，驯化伴随着同型半乳糖醛酸聚糖、木半乳糖醛酸聚糖和高度支化的鼠李半乳糖醛酸聚糖I以及支化和未支化的（1 -> 5）-α-阿拉伯聚糖和（1 -> 4）-β-半乳聚糖的增加。相比之下，敏感品种Terese积累了大量的（1 -> 4）-β-木聚糖和葡糖醛酸木聚糖，但不积累果胶。与Terese相比，在香槟中观察到更大的JIM 7标记，表明冷驯化也诱导果胶甲酯化程度的增加。冷驯化结束时，两种基因型的多聚半乳糖醛酸酶活性均显着下降。这些数据表明酯化果胶在耐寒性中的作用。讨论了果胶及其相关的阿拉伯聚糖和半乳聚糖在冷驯化中的可能功能。(C)2014爱思唯尔有限公司版权所有。
Pea (Pisum sativum) cell wall metabolism in response to chilling was investigated in a frost-sensitive genotype 'Terese' and a frost-tolerant genotype 'Champagne'. Cell walls isolated from stipules of cold acclimated and non-acclimated plants showed that cold temperatures induce changes in polymers containing xylose, arabinose, galactose and galacturonic acid residues. In the tolerant cultivar Champagne, acclimation is accompanied by increases in homogalacturonan, xylogalacturonan and highly branched Rhamnogalacturonan I with branched and unbranched (1 -> 5)-alpha-arabinans and (1 -> 4)-beta-galactans. In contrast, the sensitive cultivar Terese accumulates substantial amounts of (1 -> 4)-beta-xylans and glucuronoxylan, but not the pectins. Greater JIM7 labeling was observed in Champagne compared to Terese, indicating that cold acclimation also induces an increase in the degree of methylesterification of pectins. Significant decrease in polygalacturonase activities in both genotypes were observed at the end of cold acclimation. These data indicate a role for esterified pectins in cold tolerance. The possible functions for pectins and their associated arabinans and galactans in cold acclimation are discussed. (C) 2014 Elsevier Ltd. All rights reserved.