In vitro bone exposure to strontium improves bone material level properties
In vitro bone exposure to strontium improves bone material level properties
复制标题
DOI：
10.1016/j.actbio.2013.02.037
复制
发表时间：
2013-06-01
影响因子：
9.7
通讯作者：
Ammann, Patrick
中科院分区：
文献类型：
--
作者：
Cattani-Lorente, Maria;Rizzoli, Rene;Ammann, Patrick
在用雷奈酸锶治疗的大鼠中，完整骨的最终负荷增加，并且与微观结构和材料水平特性的变化相关。基于微计算机断层扫描的有限元分析评估表明，这些变化独立地有助于雷尼酸锶治疗引起的骨强度的改善。然而，Sr离子作用于骨材料水平特性的机制仍不清楚。将完整雌性大鼠的椎骨暴露于 0.5、1 或 2 M Sr、Ca 和 Ba 的氯化盐溶液中过夜。后两者用于评估Sr的特异性。通过在纳米压痕测试中测量硬度、弹性模量和工作能量来评估骨材料水平特性。波长色散 X 射线光谱提供半定量元素分析和绘图。与锶一起孵育使骨骼变得更硬、更硬、更坚韧。在测试的二价离子中，Sr 的影响最大。还对体内处理的骨样本进行了 Sr 亲和力评估。体外暴露后，在切除卵巢的大鼠中观察到最高的改善。然而，抗骨质疏松治疗并不影响 Sr 改变骨材料水平特性的能力。我们的研究结果表明，体外与锶一起孵育选择性地改善了骨材料水平特性，这可能有助于在锶治疗下观察到的骨特性的宏观增加。 (C) 2013 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
In rats treated with strontium ranelate, the ultimate load of intact bone is increased and associated with changes in microstructure and material level properties. Evaluation by micro-computed-tomography-based finite element analysis has shown that these changes independently contribute to the improvement of bone strength induced by strontium ranelate treatment. However, the mechanism by which Sr ion acts on bone material level properties remains unknown. The vertebrae of intact female rats were exposed overnight to 0.5, 1 or 2 M chloride salt solutions of Sr, Ca and Ba. The latter two were used to assess the specificity of Sr. Bone material level properties were evaluated by measuring hardness, elastic modulus and working energy in a nanoindentation test. Wavelength dispersive X-ray spectroscopy provided semi-quantitative elemental analysis and mapping. Incubation with Sr rendered bone stiffer, harder and tougher. Among the divalent ions tested, Sr had the greatest effect. Sr affinity was also assessed on in vivo treated bone specimens. After in vitro exposure, the highest improvements were observed in ovariectomized rats. However, anti-osteoporotic treatments did not influence the capacity of Sr to modify bone material level properties. Our findings demonstrated that in vitro incubation with Sr selectively improved bone material level properties, which may contribute to the macroscopic increase of bone properties observed under Sr therapy. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.