Gender- and thyroid hormone-related transitions of essential myosin light chain isoform expression in rat soleus muscle during ageing.
Gender- and thyroid hormone-related transitions of essential myosin light chain isoform expression in rat soleus muscle during ageing.
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衰老过程中大鼠比目鱼肌中必需肌球蛋白轻链亚型表达的性别和甲状腺激素相关转变。
DOI：
10.1046/j.1365-201x.1999.00621.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Betto,R
中科院分区：
文献类型：
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作者：
Biral,D;Ballarin,F;Toscano,I;Salviati,G;Yu,F;Larsson,L;Betto,R
本文就肌球蛋白轻链（MLC）基本亚型对肌肉细胞收缩性的调节作用作一综述。特别关注的是MLC1Sa和MLC1Sb亚型在雄性和雌性大鼠慢抽动比目鱼肌中的表达，在衰老过程中和甲状腺激素治疗后。根据二维凝胶电泳分析，在男性和女性中，MLC1Sa/MLC1SB比值随着年龄的增长而增加，与此同时，表达慢（1型）肌球蛋白重链（MHC）亚型的肌纤维缩短速度也随着年龄的增长而下降。然而，MLC1Sa和MLC1Sb异构体表达对甲状腺激素治疗有复杂的反应，这与甲状腺功能亢进动物中报道的年龄相关的缩短速度变化不同。因此，如果MLC1Sa和MLC1Sb同工异构体调节1型纤维的缩短速度，那么其他缩短速度的调节剂不受甲状腺激素与这些基本mlc协同调节。
In this brief review, the modulatory influence of essential myosin light chain (MLC) isoforms on muscle cell contractility is discussed. Specific interest is focused on the expression of the MLC1Sa and MLC1Sb isoforms in the slow‐twitch soleus muscle in male and female rats, during ageing and after thyroid hormone treatment. According to two‐dimensional gel electrophoresis analysis, the MLC1Sa/MLC1SB ratio increased during ageing in both males and females in parallel with the age‐related decrease in shortening velocity reported in muscle fibres expressing the slow (type 1) myosin heavy chain (MHC) isoform. However, the MLC1Sa and MLC1Sb isoform expression responded to thyroid hormone treatment in a complex manner which did not parallel the age‐related changes in shortening velocity reported in hyperthyroid animals. Thus, if MLC1Sa and MLC1Sb isoforms modulate shortening velocity in type 1 fibres, then other modulators of shortening velocity are not regulated by thyroid hormone in co‐ordination with these essential MLCs.