A magnetic trap for living cells suspended in a paramagnetic buffer
A magnetic trap for living cells suspended in a paramagnetic buffer
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DOI：
10.1063/1.1794372
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发表时间：
2004-09-20
影响因子：
4
通讯作者：
Prentiss, M
中科院分区：
文献类型：
--
作者：
Winkleman, A;Gudiksen, KL;Prentiss, M
这篇手稿描述了一个三维磁阱的制造和使用，用于顺磁性离子水溶液中的抗磁性物体;这个阱使用永磁体。它演示了聚苯乙烯球和各种类型的活细胞的捕获：小鼠成纤维细胞（NIH-3 T3），酵母（酿酒酵母）和藻类（莱茵衣藻）。对于在水性缓冲液中的40 mM二亚乙基三胺五乙酸钆（III）（Gd.DTPA）溶液，可以捕获的最小细胞（颗粒）具有类似于2.5 μ m的半径。被捕获的粒子和磁阱的位置可以通过独立操纵永磁体在三维中平移。该字母也描述了捕获溶液的生物相容性。(C)2004年，美国物理学会。
This manuscript describes the fabrication and use of a three-dimensional magnetic trap for diamagnetic objects in an aqueous solution of paramagnetic ions; this trap uses permanent magnets. It demonstrates trapping of polystyrene spheres, and of various types of living cells: mouse fibroblast (NIH-3T3), yeast (Saccharomyces cerevisiae), and algae (Chlamydomonas reinhardtii). For a 40 mM solution of gadolinium (III) diethylenetriaminepentaacetic acid (Gd.DTPA) in aqueous buffer, the smallest cell (particle) that could be trapped had a radius of similar to2.5 mum. The trapped particle and location of the magnetic trap can be translated in three dimensions by independent manipulation of the permanent magnets. This letter a1so characterizes the biocompatibility of the trapping solution. (C) 2004 American Institute of Physics.