Three dimensional morphology of rabies virus studied by cryo-electron tomography
Three dimensional morphology of rabies virus studied by cryo-electron tomography
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DOI：
10.1016/j.jsb.2011.07.003
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Marco, Sergio
中科院分区：
文献类型：
--
作者：
Guichard, Paul;Krell, Tino;Marco, Sergio
狂犬病病毒（RABV）仍然是一个全球性的健康问题。 RABV 含有与核蛋白 N 相关的单链 RNA 基因组。由此产生的核糖核蛋白复合物被基质蛋白 M、脂质双层和糖蛋白 G 包围。据报道，RABV 组织成子弹状病毒颗粒，但每种病毒成分在采用这种形态中的作用尚不清楚。我们在此提出了 RABV 的冷冻电子断层扫描研究，显示了由子弹状病毒粒子组成的额外形态，其中包含管状脂质附属物，其顶端具有 G 蛋白。此外，有证据表明存在重要部分的多形颗粒。这些多形性形式的膜相关 M、M/N 蛋白数量不同，这为了解其在病毒形态发生中的作用提供了有趣的见解。在没有膜相关的 M 蛋白的情况下，M/N 蛋白病毒的形态几乎是球形的。其他显示直膜部分的图像与膜上 M 蛋白的募集相关，与 G 蛋白的存在无关。病毒膜被发现含有负净电荷，表明 M-、M/N-蛋白质膜电荷吸引力驱动了这种相互作用。 (C) 2011 Elsevier Inc. 保留所有权利。
The rabies virus (RABV) continues to be a worldwide health problem. RABV contains a single-stranded RNA genome that associates with the nucleoprotein N. The resulting ribonucleoprotein complex is surrounded by matrix protein M, lipid bilayer and glycoprotein G. RABV was reported to organize in bullet-like virions, but the role of each viral component in adopting this morphology is unclear. We present here a cryo-electron tomography study of RABV showing additional morphologies consisting in bullet-like virions containing a tubular, lipidic appendage having G-protein at its apex. In addition, there was evidence for an important fraction of pleomorphic particles. These pleomorphic forms differed in the amount of membrane-associated M-, M/N-protein providing interesting insight into its role in viral morphogenesis. In the absence of membrane-associated M-, M/N-protein viral morphology was almost spherical. Other images, showing straight membrane portions, correlate with the M-protein recruitment at the membrane independently of the presence of the G-protein. The viral membrane was found to contain a negative net charge indicating that M-, M/N-protein-membrane charge attraction drives this interaction. (C) 2011 Elsevier Inc. All rights reserved.