Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion
Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion
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DOI：
10.1182/blood.v98.13.3677
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发表时间：
2001-12-15
期刊：
影响因子：
20.3
通讯作者：
Dignat-George, F
中科院分区：
文献类型：
--
作者：
Bardin, N;Anfosso, F;Dignat-George, F
CD 146是属于免疫球蛋白超家族的细胞表面分子，在所有类型的人内皮细胞中表达。共聚焦和电子显微镜分析汇合的人脐静脉内皮细胞（HUVEC）被用来证明，CD 146是内皮连接的组成部分。血管内皮钙粘蛋白双重免疫标记显示CD 146定位于粘附连接外。此外，CD 146的表达并不局限于交界处，因为在HUVEC的顶侧可检测到部分标记。有趣的是，当HUVECs达到融合时，CD 146的细胞表面表达增加。此外，与对照细胞相比，CD 146转染的成纤维细胞的细胞旁通透性降低。最后，CD 146与肌动蛋白共定位，部分耐Triton X-100提取，并通过肌动蛋白破坏剂改变其表达，表明CD 146与肌动蛋白细胞骨架相关。这些结果显示了CD 146在细胞-细胞连接区域的调节表达，并强烈表明CD 146作为细胞-细胞相互作用的介体参与其中。(C)2001年，美国血液学会。
CD146 is a cell-surface molecule belonging to the immunoglobulin superfamily and expressed in all types of human endothelial cells. Confocal and electron microscopic analysis of confluent human umbilical vein endothelial cells (HUVECs) were used to demonstrate that CD146 is a component of the endothelial junction. Double immunolabeling with vascular endothelial cadherin showed that CD146 is localized outside the adherens junction. Moreover, CD146 expression is not restricted to the junction, since part of the labeling was detectable at the apical side of the HUVECs. Interestingly, cell-surface expression of CD146 increased when HUVECs reached confluence. In addition, the paracellular permeability of CD146-transfected fibroblast cells was decreased compared with that of control cells. Finally, CD146 colocalized with actin, was partly resistant to Triton X-100 extraction, and had its expression altered by actin-disrupting agents, indicating that CD146 is associated with the actin cytoskeleton. These results show the regulated expression of CD146 at areas of cell-cell junction and strongly suggest involvement of CD146 as a mediator of cell-cell interaction. (C) 2001 by The American Society of Hematology.