Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance
Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance
复制标题
DOI：
10.1002/pmic.201200051
复制
发表时间：
2012-08-01
期刊：
影响因子：
3.4
通讯作者：
Ferro, Emer S.
中科院分区：
文献类型：
--
作者：
Berti, Denise A.;Russo, Lilian C.;Ferro, Emer S.
由蛋白酶体和寡肽酶产生的细胞内肽被认为在信号转导中起作用，并改善高热量饮食小鼠的胰岛素抵抗。本研究的目的是确定特定的细胞内肽在脂肪组织的Wistar大鼠，可能与生理和治疗控制葡萄糖摄取。使用半定量质谱和LC/MS/MS分析，我们确定了10肽在附睾脂肪组织的Wistar大鼠，这些肽中的三个是目前在增加的水平在大鼠喂食高热量的西方饮食（WD）与大鼠喂食对照饮食（CD）。亲和层析的结果表明，无论是从WD或CD大鼠附睾脂肪组织的细胞质中，独特的蛋白结合这些肽。然而，尽管在WD动物中观察到增加，但评价的肽增加了用棕榈酸酯处理的3 T3-L1脂肪细胞中胰岛素刺激的葡萄糖摄取。因此，来自Wistar大鼠脂肪组织的细胞内肽可以与特定蛋白质结合，并促进3 T3-L1脂肪细胞中胰岛素诱导的葡萄糖摄取。
Intracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high-caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high-caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin-stimulated glucose uptake in 3T3-L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin-induced glucose uptake in 3T3-L1 adipocytes.