Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells:: a global analysis of host gene regulation during infection, using a differential display approach
Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells:: a global analysis of host gene regulation during infection, using a differential display approach
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DOI：
10.1099/vir.0.19270-0
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发表时间：
2003-11-01
影响因子：
3.8
通讯作者：
Olzewski, JA
中科院分区：
文献类型：
--
作者：
Nobiron, I;O'Reilly, D;Olzewski, JA
加利福尼亚核多角体病毒（AcMNPV）是杆状病毒科的一种病毒，主要感染害虫，作为一种生物农药一直受到许多研究的关注。它也是大多数商业杆状病毒蛋白表达系统的基础。AcMNPV感染培养的宿主frugiperda （Spodoptera frugiperda, Sf9）细胞可诱导多种宿主细胞特性的改变，包括改变细胞骨架、G(2)/M细胞周期阻滞以及宿主蛋白翻译的全局关闭。此外，一些细胞转录物在AcMNPV感染后被显示下调。在这项研究中，我们采用差异显示方法来解决Sf9宿主转录本是否在感染后期发生全局下调。此外，我们也使用这种方法来搜索宿主mrna；在感染早期被上调，可能对促进杆状病毒很重要；感染。从这些实验中，我们可以确认在感染后期Sf9 mRNA水平的全局下调。我们还发现，在感染早期，个体宿主基因RNA水平的上调并不经常发生。在AcMNPV感染后，一个宿主转录物被发现是Sf9 Hsc70基因。Hsc70蛋白已被证明在其他大型DNA病毒的生命周期中起着至关重要的作用，这表明该蛋白对杆状病毒感染也很重要。
Autographa californica nucleopolyhedrovirus (AcMNPV), the type member of the virus family Baculoviridae, infects pest insects and has been the subject of many studies for its development as a biopesticide. It is also the virus upon which most of the commercial baculovirus protein expression systems are based. AcMNPV infection of cultured host Spodoptera frugiperda (Sf9) cells can induce a number of alterations of host cell properties including altering the cellular cytoskeleton, an arrest of the cell cycle in G(2)/M, and the global shutoff of host protein translation. Additionally, several cellular transcripts have been shown to be down-regulated following AcMNPV infection. In this study, we take a differential display approach to address whether a global down-regulation of Sf9 host transcripts occurs at late times of infection. Additionally, we also use this approach to search for host mRNAs; which are up-regulated at early times of infection, and may be important for facilitating baculovirus; infection. From these experiments we can confirm a global down-regulation of Sf9 mRNA levels at late times of infection. We also found that up-regulation of individual host gene RNA levels at early times of infection did not occur frequently. One host transcript which was found to be transiently up-regulated as a result of AcMNPV infection was an Sf9 Hsc70 gene. Hsc70 proteins have been shown to play a vital role in the life-cycle of other large DNA viruses, which suggests that this protein is also important for baculovirus infection.