Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.
Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.
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DOI：
10.4103/0973-6247.59386
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发表时间：
2010-01
影响因子：
--
通讯作者：
Viswanathan C
中科院分区：
文献类型：
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作者：
Shetty P;Cooper K;Viswanathan C
造血干细胞（Hematopoietic stem cells，HSC）和间充质干细胞（mesenchymal stem cells，MSCs）是目前研究最多的两种成体干细胞。到目前为止，MSC是从骨髓中获得的，这是一个痛苦的过程。因此，来自不太常见来源的MSC，如脐带血、脂肪组织、牙髓等，一直是研究的主题。本研究的目的是探索从争议较少，容易和丰富的来源（如脐带）中找到MSC的可能性，以用于潜在的再生医学应用。本研究使用了5份骨髓样本（BM）、70份脐带血单位（CB）和4份脐带基质（CM）样本。通过流式细胞术检查扩增的MSC的生物标志物表达，并且还检查它们向中胚层和外胚层谱系的分化。与仅6%的CB样品相比，可以从100%的BM和CM样品中分离MSC。与BM（BM-MSC）和CM（CM-MSC）相比，在CB（CB-MSC）中观察到的间充质干细胞的倍数扩增明显更高。从所有三种来源分离的MSC表达特征性间充质表型CD 45 − /vWF − /CD 14 − /CD 31 − /CD 73 + /CD 105 + /SSEA 4 + /CD 29 + /CD 44 + /HLAABC +，而CM-MSC和CB-MSC中明显缺乏HLA DR。尽管在所有来源的MSC中都观察到成骨、成软骨和神经分化，但仅在BM-MSC中观察到成脂分化。CM-MSC是再生医学中自体和同种异体使用的无限数量MSC的可靠来源。
Hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) are the two widely studied and characterized adult stem cells. Thus far, MSCs were obtained from the bone marrow, which is a painful procedure. Therefore, MSCs from less common sources like cord blood, adipose tissue, tooth pulp, and so on, have been the subject of research. The purpose of this study is to explore the possibility of finding MSCs from a less controversial, easy, and abundant source, such as the umbilical cord, for potential regenerative medicine applications. Five bone marrow samples (BM), seventy cord blood units (CB), and four umbilical cord matrix (CM) samples have been used for the study. Expanded MSCs were checked for biomarker expression by flow cytometry and were also checked for their differentiation to mesodermal and ectodermal lineages. MSCs could be isolated from 100% BM and CM samples, as compared to only 6% of CB samples. The fold expansion of the mesenchymal stem cells observed in CB (CB-MSCs) was distinctly higher as compared to BM (BM-MSCs) and CM (CM-MSCs). MSCs isolated from all the three sources expressed a characteristic mesenchymal phenotype of CD45 − /vWF − /CD14 − /CD31 − /CD73 + /CD105 + /SSEA4 + /CD29 + /CD44 + /HLAABC +, whereas, the HLA DR was conspicuously absent in CM-MSCs and CB-MSCs. Although osteogenic, chondrogenic, and neural differentiation was observed in MSCs from all sources, adipogenic differentiation was observed only in BM-MSCs. CM-MSCs are a dependable source of an unlimited number of MSCs for autologous and allogenic use in regenerative medicine.