Macromolecular crystal annealing: Overcoming increased mosaicity associated with cryocrystallography
Macromolecular crystal annealing: Overcoming increased mosaicity associated with cryocrystallography
复制标题
DOI：
10.1107/s0907444997019008
复制
发表时间：
1998-07-01
影响因子：
2.2
通讯作者：
Bunick, GJ
中科院分区：
文献类型：
--
作者：
Harp, JM;Timm, DE;Bunick, GJ
尽管低温数据收集已成为大分子晶体学的首选方法，但快速冷却步骤可以显着增加某些晶体的镶嵌性。大分子晶体退火显着降低了闪冷晶体的镶嵌性而不影响分子结构。该过程将闪冷晶体循环至环境温度并返回低温，简单、快速且不需要特殊设备。退火过程已应用于由不同沉淀剂和使用各种冷冻保护剂生长的几种不同大分子的晶体。大分子晶体退火方案也已应用于恢复在转移至低温存储或从低温存储转移过程中处理不当的快速冷却晶体的衍射。这些结果将结合散列冷却晶体中的晶体镶嵌性和辐射损伤的影响进行讨论。
Although cryogenic data collection has become the method of choice for macromolecular crystallography, the flash-cooling step can dramatically increase the mosaicity of some crystals. Macromolecular crystal annealing significantly reduces the mosaicity of flash-cooled crystals without affecting molecular structure. The process, which cycles a flash-cooled crystal to ambient temperature and back to cryogenic temperature, is simple, quick and requires no special equipment. The annealing process has been applied to crystals of several different macromolecules grown from different precipitants and using a variety of cryoprotectants. The protocol for macromolecular crystal annealing also has been applied to restore diffraction from flash-cooled crystals that were mishandled during transfer to or from cryogenic storage. These results will be discussed in relation to crystal mosaicity and effects of radiation damage in hash-cooled crystals.