T-cell receptor TCRG1 and TCRG2 clusters map separately in two different regions of sheep chromosome 4
T-cell receptor TCRG1 and TCRG2 clusters map separately in two different regions of sheep chromosome 4
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DOI：
10.1023/a:1009245830804
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发表时间：
1998-08-01
影响因子：
2.6
通讯作者：
Ciccarese, S
中科院分区：
文献类型：
--
作者：
Massari, S;Lipsi, MR;Ciccarese, S
t细胞受体（TCR）复合物是由α- β-链（TCRA/TCRB复合物）或γ-链（TCRG/TCRD复合物）组成的异源二聚体。人类和小鼠的TCRA、TCRB、TCRG和TCRD基因的基因组组织及其染色体定位已被确定（O'Brien 1993）。偶蹄动物是大型哺乳动物中辐射最剧烈的一目，TCRG/TCRD复合体是外周TCR池的主要形式（Hein et al. 1993）。然而，关于这些基因在这些物种中的基因组组织和染色体定位的信息很少。最近，在绵羊中，TCRA/TCRD位点被分配到染色体带7q1。4±q2。2 （Massari et al. 1997）。此外，对γδ TCR cdna的分析表明，绵羊基因组含有5个功能性的Cγ片段（Cγ1±Cγ5）（Hein et al. 1993）。本文报道了含有c - γ1和c - γ2恒定基因的绵羊基因组克隆的分离、分子鉴定和细胞学定位。
T-cell receptor (TCR) complex is a heterodimer composed of an α-and a β-chain (TCRA/TCRB complex) or a γ-and a δ-chain (TCRG/TCRD complex). The genomic organization of TCRA, TCRB, TCRG and TCRD genes and their chromosomal localization have been determined in human and mouse (O'Brien 1993). In artiodactyls, an order representing the most dramatic radiation of large mammals, the TCRG/TCRD complex represents the predominant form of the peripheral TCR pool (Hein et al. 1993). However, little information is available on the genomic organization and chromosomal localization of these genes in these species. Recently, in sheep, the TCRA/TCRD locus has been assigned to chromosome bands 7q1. 4±q2. 2 (Massari et al. 1997). Moreover, analysis of γδ TCR cDNAs indicates that the sheep genome contains® ve functional Cγ segments (Cγ1±Cγ5)(Hein et al. 1993). In the present paper, we report isolation, molecular characterization and cytological mapping of sheep genomic clones containing Cγ1 and Cγ2 constant genes.