Effects of Srxn1 on growth and Notch signalling of astrocyte induced by hydrogen peroxide
Effects of Srxn1 on growth and Notch signalling of astrocyte induced by hydrogen peroxide
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DOI：
10.1080/21691401.2019.1614016
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Ni, Changwei
中科院分区：
文献类型：
--
作者：
Li, Lan;Lin, Guangjun;Ni, Changwei
目的：目的：探讨硫氧还蛋白-1（Sulfiredoxin-1，Srxn 1）对过氧化氢（H2 O2）诱导的星形胶质细胞损伤的影响。结果：Srxn 1基因敲减可促进H2 O2诱导的星形胶质细胞LDH和MDA的分泌，降低SOD活性，加重H2 O2诱导的星形胶质细胞凋亡。Western blot、ELISA和流式细胞仪检测结果表明，激活Notch信号通路可减弱Srxn 1对H2 O2诱导的星形胶质细胞氧化损伤和凋亡的影响。结论：Srxn 1可能通过激活Notch信号通路对H2 O2诱导的星形胶质细胞氧化应激损伤具有保护作用。
Objective: To investigate the effect of Sulfiredoxin-1 (Srxn1) on astrocyte injury induced by hydrogen peroxide (H2O2).Methods: Observing the changes of H2O2 on contents of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) and apoptosis after transfected Srxn1 siRNA into astrocytes. The protein expression of Notch 1, NICD and Hest, the content of LDH and MDA, the activity of SOD and apoptosis rate of astrocytes after inhibiting or activation of Notch signalling pathway were detected by Western blot, ELISA and flow cytometry, respectively.Results: Knockdown of Srxn1 could promote the secretion of LDH and MDA, decrease the activity of SOD and aggravate apoptosis of astrocytes induced by H2O2. The results of Western blot, ELISA assay and flow cytometry indicated that activation of the Notch signalling pathway attenuated the effect of Srxn1 on H2O2-induced oxidative damage and apoptosis of astrocytes.Conclusion: Srxn1 may protect astrocytes from oxidative stress injury induced by H2O2 by activation of Notch signalling pathway.