Diversity of insect intestinal microflora
Diversity of insect intestinal microflora
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DOI：
10.1007/s12223-008-0032-z
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发表时间：
2008-07-01
影响因子：
2.6
通讯作者：
Kopecny, J.
中科院分区：
文献类型：
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作者：
Mrazek, J.;Strosova, L.;Kopecny, J.
使用 DGGE 分析评估了蜜蜂、黄蜂和蟑螂肠道微生物区系的地理位置、季节、年龄和消化道部位对细菌多样性的影响。使用针对 16S rDNA 基因 200 bp 区域的通用细菌引物进行 PCR-DGGE 分析，提供了复杂细菌 DNA 的概况；使用特异性引物来确定双歧杆菌的概况，通过实时PCR确定双歧杆菌在消化道中的浓度。通过测序鉴定选定的 PCR 产物。远距离蜂巢中蜜蜂的微生物区系几乎没有变化。它们的双歧杆菌种群占细菌总数的 2.8-8.4%，并且非常均匀。黄蜂的肠道菌群总量也很均匀，只有两个样本受到季节或地点的影响；另一方面，黄蜂双歧杆菌种群非常异质。蟑螂的微生物群组成差异最大，年龄和饮食是最终因素；双歧杆菌计数在测试个体之间也存在差异（占细菌总数的 0.1-34.1%）。我们的结果表明，营养习惯是影响昆虫微生物区系的最强因素，给杂食性物种带来更大的变异。
The influence of geographic location, season, age, and part of the digestive tract on bacterial diversity was evaluated on intestinal microflora of honeybees, wasps, and cockroaches using DGGE analysis. PCR-DGGE analyses with universal bacterial primers targeting 200-bp region of the 16S rDNA gene afforded the profile of complex bacterial DNA; specific primers were used to determine the profile of bifidobacteria whose concentration in digestive tract was determined by real-time PCR. Selected PCR products were identified by sequencing. The microflora of the bees exhibited little variations among the hives from distant locations. Their bifidobacterial population formed 2.8-8.4 % of total bacteria and was very homogeneous. The total gut microflora of wasps was also homogeneous, only two samples being affected by the season or the location; on the other hand, wasp bifidobacterial population was very heterogeneous. Cockroaches showed the highest variations in microflora composition, the age and diet being the ultimate factors; bifidobacteria counts also varied among tested individuals (0.1-34.1 % of total bacteria). Our results suggest that nutrition habits are the strongest factor affecting the insect microflora, giving higher variations to omnivorous species.