The genetic control of plant mitochondrial morphology and dynamics
The genetic control of plant mitochondrial morphology and dynamics
复制标题
DOI：
10.1046/j.1365-313x.2003.01894.x
复制
发表时间：
2003-11-01
期刊：
影响因子：
7.2
通讯作者：
Tobin, AK
中科院分区：
文献类型：
--
作者：
Logan, DC;Scott, I;Tobin, AK
关于高等植物线粒体形态和动力学的遗传控制知之甚少。我们使用遗传筛选，对表达针对线粒体的 GFP 的甲基磺酸乙酯 (EMS) 突变拟南芥幼苗进行荧光显微镜分析，以分离出八个突变体，这些突变体显示出正常线粒体形态或分布的明显扰动。我们描述了具有独特线粒体表型的五种突变体，它们映射到五个不同的位点，以前与植物中的线粒体行为无关。我们利用正向和反向遗传学的组合来鉴定其中一个基因，友好线粒体（FMT），它是盘基网柄菌 CluA 基因的同源物，它参与线粒体在细胞中的正确分布。这五个突变体构成了帮助我们了解高等植物线粒体动力学的强大资源。
Little is known about the genetic control of mitochondrial morphology and dynamics in higher plants. We used a genetic screen involving fluorescence microscopic analysis of ethyl methane sulphonate (EMS)-mutated Arabidopsis thaliana seedlings expressing GFP targeted to mitochondria to isolate eight mutants displaying distinct perturbations of the normal mitochondrial morphology or distribution. We describe five mutants with distinct and unique mitochondrial phenotypes, which map to five different loci, not previously implicated in mitochondrial behaviour in plants. We have used a combination of forward and reverse genetics to identify one of the genes, friendly mitochondria (FMT), a homologue of the CluA gene of Dictyostelium discoideum, which is involved in the correct distribution of mitochondria in the cell. The five mutants constitute a powerful resource to aid our understanding of mitochondrial dynamics in higher plants.