Regional effects of ethanol on glutamate levels, uptake and release in slice and synaptosome preparations from rat brain.
Regional effects of ethanol on glutamate levels, uptake and release in slice and synaptosome preparations from rat brain.
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乙醇对大鼠脑切片和突触体制剂中谷氨酸水平、摄取和释放的区域影响。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
K. von Hungen
中科院分区：
文献类型：
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作者：
E. Keller;J. Cummins;K. von Hungen
慢性乙醇处理影响K+刺激的内源性谷氨酸释放减少和大鼠额叶皮层谷氨酸水平增加。这种处理也增加了纹状体和海马切片中的（3 H）-谷氨酸摄取。在皮质切片或突触体中均未观察到摄取变化。乙醇戒断增加皮层和海马中K+刺激的谷氨酸释放以及皮层、纹状体和海马中的谷氨酸水平。在戒断动物的纹状体中（3 H）-谷氨酸摄取增加。因此，在影响和影响程度方面都观察到了区域差异。因为在体外和急性乙醇治疗没有影响的吸收或释放，这些变化可能是由于激活的反馈机制，试图补偿乙醇的行动对大脑系统，而不是直接影响膜结构。结合水平释放数据表明乙醇对皮质和纹状体谷氨酸分布的影响。结合摄取和释放数据表明减少谷氨酸突触活动和成瘾状态之间的相关性，以及谷氨酸突触活动和撤回状态之间的相关性。
Chronic ethanol treatment effects a decrease in K+-stimulated endogenous glutamate release and an increase in glutamate levels in rat frontal cortex. This treatment also increases (3H)-glutamate uptake in striatal and hippocampal slices. No uptake changes were observed in either cortical slices or synaptosomes. Ethanol withdrawal increases K+-stimulated glutamate release in cortex and hippocampus and glutamate levels in cortex, striatum and hippocampus. (3H)-glutamate uptake is increased in striatum of withdrawn animals. Thus, regional variations were observed with regard to both effect and degree of effect. Because in vitro and acute ethanol treatments had no effect on uptake or release, these changes probably result from activated feedback mechanisms that attempt to compensate for ethanol's action on brain systems rather than direct effects on membrane structure. Combined level-release data suggest an effect of ethanol on glutamate distribution in cortex and striatum. Combined uptake and release data indicate a correlation between diminished glutamate synaptic activity and the addicted state, and between heightened glutamate synaptic activity and the withdrawn state.