PINOCYTOSIS IN RAT VISCERAL YOLK-SAC - EFFECTS OF TEMPERATURE, METABOLIC-INHIBITORS AND SOME OTHER MODIFIERS
PINOCYTOSIS IN RAT VISCERAL YOLK-SAC - EFFECTS OF TEMPERATURE, METABOLIC-INHIBITORS AND SOME OTHER MODIFIERS
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DOI：
10.1016/0304-4165(78)90339-2
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
LLOYD, JB
中科院分区：
文献类型：
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作者：
DUNCAN, R;LLOYD, JB
低温、2，4-二硝基苯酚和一碘乙酸均能完全阻断体外培养17.5天的大鼠卵黄囊对125 I标记的聚乙烯吡咯烷酮、125 I标记的牛血清白蛋白和胶体198 Au的吞饮摄取。细胞松弛素B和秋水仙碱引起部分和剂量依赖性抑制。这些底物的胞饮摄取机制显然不是传统定义的微胞饮作用。细胞外钙的去除或茶碱的存在抑制液相胞饮作用的大鼠卵黄囊，而哇巴因的加入引起的双相反应：在低浓度和在较高浓度的抑制作用的胞饮体形成的轻微刺激。
Low temperature, 2,4-dinitrophenol and moniodoacetate could each completely abolish the pinocytic uptake of 125I-labelled polyvinylpyrrolidone, 125I-labelled bovine serum albumin or colloidal 198Au by 17.5-day rat visceral yolk sac cultured in vitro. Cytochalasin B and colchicine caused a partial and dose-dependent inhibition. The mechanism of pinocytic uptake of these substrates apparently is not micropinocytosis as conventionally defined. Removal of extracellular Ca or the presence of theophylline inhibited liquid-phase pinocytosis by the rat yolk sac, whereas addition of ouabain caused a biphasic response: a slight stimulation of pinosome formation at a low concentration and an inhibitory effect at a higher concentration.