Improved technique for cannulation of the murine thoracic duct: A valuable tool for the dissection of immune responses
Improved technique for cannulation of the murine thoracic duct: A valuable tool for the dissection of immune responses
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DOI：
10.1016/s0022-1759(96)00226-8
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发表时间：
1997-03-10
影响因子：
2.2
通讯作者：
Solbach, W
中科院分区：
文献类型：
--
作者：
Ionac, M;Laskay, T;Solbach, W
过去已经使用了几种实验方法用于小鼠胸导管插管。然而，大多数的特点是适度的可重复性和低淋巴产量。在这里，我们描述了一种插管技术的麻醉方面的修改，使用硅胶套管和一个简单而有效的腹腔内固定的排水。手术平均45分钟，术中和术后死亡率为零。手术后，小鼠可以使用运动轮，从而增加活动性并因此获得良好的淋巴液流动，从而保持插管的功能。小鼠每24小时产生平均29.3 ml（范围8-40 ml）胸导管淋巴液，其中在前23小时内平均含有2.2 x 10（6）个淋巴细胞/ml，在插管后第2天降至0.1 x 10（6）个淋巴细胞/ml。3天后插管通畅率为100%。有趣的是，我们已经检测到小鼠胸导管解剖结构的应变依赖性差异，并且当尝试插管手术时需要考虑这些差异。
Several experimental approaches have been used in the past for the cannulation of the thoracic duct in mice. Most, however, are characterized by a modest reproducibility and a low lymph yield. Here, we describe a cannulation technique modified with respect to the anesthesia, the use of a silicone cannula and a simple and efficient intraabdominal fixation of the drain. Surgery averaged 45 min with an intra- and postoperative mortality rate of zero. Postoperatively, mice were given access to an exercise wheel allowing increased mobility and consequently a good lymph flow, thus maintaining the function of the cannula. The mice yielded a mean of 29.3 ml/24 h (range 8-40 ml) thoracic duct lymph, which contained a mean of 2.2 x 10(6) lymphocytes/ml during the first 23 h, decreasing to 0.1 x 10(6) lymphocytes/ml on the 2nd day after cannulation. Patency of the cannulae was 100% after 3 days. Interestingly, we have detected strain dependent differences in the anatomy of the thoracic duct in the mouse and these need to be considered when cannulation procedures are attempted.