Are heterogenous results of EGFR immunoreactivity in renal cell carcinoma related to non-standardised criteria for staining evaluation?
Are heterogenous results of EGFR immunoreactivity in renal cell carcinoma related to non-standardised criteria for staining evaluation?
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DOI：
10.1136/jcp.2003.015743
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发表时间：
2004-07-01
影响因子：
3.4
通讯作者：
Zigeuner, R
中科院分区：
文献类型：
--
作者：
Langner, C;Ratschek, M;Zigeuner, R
目的：评估肾细胞癌（RCC）中表皮生长因子受体（EGFR）免疫反应性的异质性是否与染色评估的非标准化标准有关。方法：利用组织芯片技术研究了132例原发性和55例转移性常规rcc的EGFR表达。结果：总体而言，132例原发性rcc中有123例（93%）和53例转移性rcc中有49例（92%）存在膜性和/或细胞质性EGFR免疫染色，132例原发性肿瘤中有110例（83%）和53例转移性肿瘤中有39例（73%）存在广泛的免疫反应性（50%的肿瘤细胞）。细胞质染色与高肿瘤分期和高肿瘤分级相关。此外，高级别rcc中存在强烈的膜性染色（评分3+）。细胞质免疫染色与不良预后相关，而总体（细胞质和膜性）免疫反应性和膜性染色强度与不良预后无关。结论：不同的免疫组织化学评估方法导致不同的结果，加强了标准化的必要性，特别是在快速发展的EGFR靶向癌症治疗策略的背景下。
Aims: To assess whether heterogeneity of epidermal growth factor receptor ( EGFR) immunoreactivity in renal cell carcinoma (RCC) is related to non-standardised criteria for staining evaluation.Methods: EGFR expression was investigated in 132 primary and 55 metastatic onventional RCCs using a tissue microarray technique.Results: Overall, membranous and/or cytoplasmic EGFR immunostaining was present in 123 of 132 (93%) primary and 49 of 53 (92%) metastatic RCCs, with extensive immunoreactivity (>50% of tumour cells) in 110 of 132 (83%) primary tumours and 39 of 53 (73%) metastases. Cytoplasmic staining was associated with high tumour stage and high tumour grade. In addition, strong membranous staining ( score 3+) prevailed in high grade RCCs. Cytoplasmic immunostaining was associated with an unfavourable prognosis, whereas overall ( cytoplasmic and membranous) immunoreactivity and intensity of membranous staining were not.Conclusions: Different methods of immunohistochemical evaluation led to different results, strengthening the need for standardisation, especially against a background of rapidly evolving EGFR targeted cancer treatment strategies.