The Arabidopsis thaliana transcriptome in response to Agrobacterium tumefaciens
The Arabidopsis thaliana transcriptome in response to Agrobacterium tumefaciens
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DOI：
10.1094/mpmi-19-0665
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发表时间：
2006-06-01
影响因子：
3.5
通讯作者：
Nester, Eugene W.
中科院分区：
文献类型：
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作者：
Ditt, Renata F.;Kerr, Kathleen F.;Nester, Eugene W.
病原体根癌农杆菌感染广泛的植物，将T-DNA引入它们的基因组中。与所有已知的植物病原细菌相反，A.根癌农杆菌缺乏诱导过敏反应的hrp基因，尽管它通过IV型分泌系统将许多蛋白质引入植物细胞。为了了解植物对这种不寻常的病原体的转录反应的时间和程度，我们使用了拟南芥26，000个基因的寡核苷酸微阵列。我们用致癌农杆菌菌株接种拟南芥细胞培养物，并分析了四个生物学重复，以确定两组强大的调控基因，一组诱导，另一组抑制。在这两种情况下，在感染后48小时的反应是明显的，但不是在24小时或更早。诱导的集合包括编码已知防御蛋白的基因，而抑制的集合富含细胞增殖的基因特征，即使在接种的培养物中看不到生长停滞。对抑制基因的分析揭示了保守的上游调控元件Frankiebox（也称为“位点II”）和Telobox与基因表达的抑制相关。调控基因集应该是有用的解剖在这种植物-病原体相互作用的信号通路。
The pathogen Agrobacterium tumefaciens infects a broad range of plants, introducing the T-DNA into their genome. Contrary to all known bacterial phytopathogens, A. tumefaciens lacks the hypersensitive response-inducing hrp genes, although it introduces numerous proteins into the plant cell through a type IV secretion system. To understand the timing and extent of the plant transcriptional response to this unusual pathogen, we used an Arabidopsis 26,000-gene oligonucleotide microarray. We inoculated Arabidopsis cell cultures with an oncogenic Agrobacterium strain and analyzed four biological replicates to identify two robust sets of regulated genes, one induced and the other suppressed. In both cases, the response was distinct at 48 h after infection, but not at 24 It or earlier. The induced set includes genes encoding known defense proteins, and the repressed set is enriched with genes characteristic of cell proliferation even though a growth arrest was not visible in the inoculated cultures. The analysis of the repressed genes revealed that the conserved upstream regulatory elements Frankiebox (also known as "site II") and Telobox are associated with the suppression of gene expression. The regulated gene sets should be useful in dissecting the signaling pathways in this plant-pathogen interaction.